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was prepared fresh on the day of use. Infrequently, a
batch of o-phthalaldehyde was receivedfrom the supplier
which didnot give reproducible color development;
however, this was true for less than 10% of the shipments
obtained. Most of the o-phthalaldehyde purchased had
a shelflife of at least 6 months. FeC13was purchased
from Fisher Scientific Co., Ltd.,Montreal, Canada.
Determination of cholesterol
A stock solution was prepared by dissolving 840 mg of
using o-phthalaldehyde FeCla. 6Hz0 in10 ml of glacial acetic acid. A work
solution was prepared by making a 1:100 dilution of
L. L. RUDELAND M. D. MORRIS stockFeCla with glacial acetic acid. The work FeCl,
Banting and Best Department of Medical Research, was prepared 1day prior to use and was stable for several
University of Toronto,Toronto, Canada, and months.
Departments of Biochemistry and Pediatrics, University Proced
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