Multiwavelength Fluorescence Detection For Dna Sequencing Using Capillary Electrophoresis英文电子书.pdf

k.) 1991 Oxford University Press Nucleic Acids Research, Vol. 19, No. 18 4955-4962 Multiwavelength fluorescence detection for DNA sequencing using capillary electrophoresis Achim E.Karger, Joel M.Harris1 and Raymond F.Gesteland University of Utah, Department of Human Genetics and 1Department of Chemistry, Salt Lake City, UT 84112, USA Received June 10, 1991; Revised and Accepted July 29, 1991 ABSTRACT Multiwavelength detection of laser induced high speed, improved electrophoretic separation, improved fluorescence for dideoxynucleotide DNA sequencing sensitivity of the fluorescence detection and a potential for with four different fluorophores and separation by automation when compared to slab gel separation[7- 12]. High- capillary gel electrophoresis is described. A speed separation and simultaneous fluorescence detection of the cryogenically cooled, low readout noise, 2-dimensional four different dye labels for DNA sequencing with capillary charge-coupled device is used as a detector for the on- electrophoresis using photomultiplier (PMT)detectors has been line, on-column recording of emission spectra. The described[9,12]. H.Swerdlow and N.Dovichi have investigated detection system has no moving parts and provides two different sets of fluorophores and the benefits of a sheath wavelength selectivity on a single detector device. The flow detector cell for fluorescent DNA sequencing[12]. detection limit of fluorescently labeled oligonucleotides In all the published fluorescent detection systems for slab and meets the high sensitivity requirements for capillary capillary gel sequencing, wavelength selectivity is obtained by DNA sequencing largely due to the efficient operation positioning band