Quercetin induced apoptosis in association with death receptors and fludarabine in cells isolated from chronic lymphocytic leukaemia patients英文电子书.pdf

British Journal of Cancer (2010) 103, 642 – 648 2010 Cancer Research UK All rights reserved 0007 – 0920/10 Quercetin induced apoptosis in association with death receptors and fludarabine in cells isolated from chronic lymphocytic leukaemia patients 1 1 2 1 1 ,1 * M Russo , C Spagnuolo , S Volpe , A Mupo , I Tedesco and G-L Russo 1 2 Institute of Food Sciences, National Research Council, Avellino 83100, Italy; Onco-Haematology Division, SG Moscati Hospital, Avellino 83100, Italy T BACKGROUND: Quercetin is a flavonoid naturally present in food and beverages belonging to the large class of phytochemicals with r potential anti-cancer properties. Here, we investigated the ability of quercetin to sensitise primary cells from chronic lymphocytic a n leukaemia (CLL) to death receptor (DR) agonists, recombinant TNF-related-apoptosis-inducing ligand (rTRAIL) and anti-CD95, and s l to fludarabine, a widely used chemotherapeutic drug against CLL. a t METHODS: Peripheral white blood cells were isolated from patients and incubated with medium containing 50 ng ml anti-CD95 agonist i o antibody; 10 ng ml recombinant TRAIL; 10–25 mM quercetin and 3.5–14 mM fludarabine. Neutral Red assay was used to measure cell n a viability, where as apoptosis was assessed by determining caspase-3 activity, exposure to Annexin V and PARP fragmentation. l