FLICE, A Novel FADD Homologous ICE CED 3–like Protease, Is Recruited to the CD95 (Fas APO 1) Death Inducing Signaling Complex英文电子书.pdf

Cell, Vol. 85, 817–827, June 14, 1996, Copyright 1996 by Cell Press FLICE, A Novel FADD-Homologous ICE/CED-3–like Protease, Is Recruited to the CD95 (Fas/APO-1) Death-Inducing Signaling Complex Marta Muzio,* Arul M. Chinnaiyan,* have been identified, the way in which they interact to Frank C. Kischkel,† Karen O’Rourke,* execute the apoptotic program is poorly understood. Andrej Shevchenko,‡ Jian Ni,§ Carsten Scaffidi,† It is becoming apparent that cysteine proteases re- James D. Bretz,* Mei Zhang,§ Reiner Gentz,§ lated to the Caenorhabditis elegans cell death gene Matthias Mann,‡ Peter H. Krammer,† ced-3 represent the effector components of the apop- Marcus E. Peter,†# and Vishva M. Dixit*# totic machinery. The first mammalian homolog of CED-3 *University of Michigan Medical School identified was interleukin-1–converting enzyme (ICE; Department of Pathology Yuan et al., 1993). Overexpression of ICE or CED-3 in Ann Arbor, Michigan 48109 Rat-1 fibroblasts induced apoptosis, suggesting that †Tumorimmunology Program ICE was functionally, as well as structurally, related to German Cancer Research Center CED-3 (Miura et al., 1993). However, such evidence is Im Neuenheimer Feld 280 only a correlation, as ectopic expression of a number 69120 Heidelberg of proteases, including chymotrypsin, proteinase K, and Federal Republic of Germany trypsin, cause significant apoptosis (Williams and Henk- ‡ Protein