Cloning, Sequence, And Expression Of The Drosophila Camp Dependent Protein Kinase Catalytic Subunit Gene英文电子书.pdf

THEJOURNAL OF BIOLOGICALCHEMISTRY Vol. 263, No. 4, Issue of February 5,pp. 1676-1681, 1988 Printed in U.S.A. Cloning, Sequence, and Expression of the Drosophila CAMP-dependent Protein Kinase Catalytic SubunitGene* (Received for publication, April 20, 1987) John L. Foster$§, Gloria C. Higgins$, and F. Rob Jacksonnll From the $Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892 and llThe Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545 Genomic DNA containing the proteincoding region this formof CAMP-dependent protein kinase(which has been for Drosophila CAMP-dependent protein kinase cata- designated type IIA, see Ref. 2) was the predominant form lytic subunit hasbeen clonedand sequenced. The probe expressed in adults. Evidence of additional forms of type I1 used to detect and isolate the gene fragment wascon- regulatory subunit (2-4) was not found inDrosophila, and the structed from two partially complementary synthetic type I CAMP-dependent proteinkinase seemed to be co- 60 base pairs thatencode oligonucleotides and contains expressed only in larvae (1).This fortuitous situation in the (using Drosophila codon preferences)aminoacids adult fly facilitatedgenetic studies of thetype I1 CAMP- 195-2 14of the beef heart ca