Biomatrix Polymer Composite Material for Heart Valve Tissue Engineering英文电子书.pdf

Biomatrix/Polymer Composite Material for Heart Valve Tissue Engineering C A R D Christof Stamm, MD, Amir Khosravi, MD, Niels Grabow, MS, Kathleen Schmohl, PhD, I O Nadine Treckmann, BS, Anne Drechsel, BS, Ma Nan, PhD, Klaus-Peter Schmitz, PhD, V A S Axel Haubold, PhD, and Gustav Steinhoff, MD C U L Department of Cardiac Surgery, Institute for Biomedical Engineering, Research Center for Cardiac Tissue Replacement, University A R of Rostock, Rostock, Germany Background. Decellularized extracellular matrix has Results. Biocompatibility assays indicated that human been suggested as a scaffold for heart valve tissue engi- blood vessel cells survive and proliferate on matrix/ neering or direct implantation. However, cell removal polymer hybrid tissue. In vitro activation of cellular and impairs the physical properties of the valve structure and plasmatic coagulation cascades was lower than with exposes bare collagen fibers that are highly thrombo- uncoated control tissue. After implantation in the rabbit genic. Matrix/polymer hybrid valves with improved bio- aorta, matrix/polymer hybrid patches healed well, with logical and mechanical characteristics may be complete endothelialization, mild leukocyte infiltration, advantageous. and less calcification than control tissue. Matrix/polymer Methods. Porcine aortic valves were decellularized hybrid tissue had superior tensile strength and suture enzymatically and impregnated with biodegradable retention strength, and hybrid valves showed good fluid poly(hydroxybutyrate) by a