Autolysis of cell walls from polygalacturonase antisense tomato fruit in simulated apoplastic solutions英文电子书.pdf

Plant Physiology and Biochemistry 49 (2011) 617e622 Contents lists available at ScienceDirect Plant Physiology and Biochemistry journal homepage: www.else /loc ate/plaphy Research article Autolysis of cell walls from polygalacturonase-antisense tomato fruit in simulated apoplastic solutions Domingos P.F. Almeida a, b, *, Donald J. Huber c a Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal b CBQF, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal c Horticultural Sciences Department, Institute of Food and Agricultural Sciences, PO Box 110690, University of Florida, Gainesville, FL 32611, USA a r t i c l e i n f o a b s t r a c t Article history: Autolysis of cell walls from polygalacturonase (PG)-antisense tomato fruit was studied in a conventional Received 17 February 2010 buffer designed to maximize the catalytic activity of PG (30 mM sodium acetate, 150 mM NaCl, pH 4.5), Accepted 23 February 2011 and in solutions mimicking the pH and mineral composition of the fruit apoplast at the mature-green Available online 3 March 2011 and ripe stages. Autolytic release of uronic acids was very limited under simulated apoplastic conditions compared with the conventional buffer, but minimal differences in the release of reducing groups were Keywords: observed among the incubation conditions. Autolytic release of uronic acids from a