Simultaneous analysis of ochratoxin A and its major metabolite ochratoxin alpha in plasma and urine for an advanced biomonitoring of the mycotoxin英文电子书.pdf

G Model CHROMB-16667; No. of Pages 7 ARTICLE IN PRESS Journal of Chromatography B, xxx (2009) xxx–xxx Contents lists available at ScienceDirect Journal of Chromatography B j o u rn al h omepage: w w w. elsev /locate/chro mb Simultaneous analysis of ochratoxin A and its major metabolite ochratoxin alpha in plasma and urine for an advanced biomonitoring of the mycotoxin, ∗ ˜ Katherine Munoz , Meinolf Blaszkewicz, Gisela H. Degen Leibniz Research Centre for Working Environment and Human Factors, Ardeystraße 67, D-44139 Dortmund, Germany a r t i c l e i n f o a b s t r a c t Article history: Ochratoxin A (OTA) is a frequent mycotoxin contaminant found worldwide in foods and feedstuffs. Received 28 August 2009 Biomonitoring has been used to assess internal OTA exposure resulting from dietary intake and from Accepted 26 November 2009 other sources. Mycotoxin levels in blood and/or urine provide good estimates of past and recent expo- Available online xxx sure since OTA binds to serum proteins and is also partly excreted via the kidney. But, measuring OTA alone does not reflect its biotransformation. In light of scarce data on its metabolites in humans, it was Keywords: the aim of this study to develop a method that allows analysis of OTA and its detoxication product ochra- Mycotoxins toxin alpha (OT) in