Insulin like Growth Factor independent Action of IGF binding Protein 3 in Hs578T Human Breast Cancer Cells英文电子书.pdf

THEJOURNALOF BIOLOGICALCHEMISTRY Val. 268, No. 20, Issue of July 15, pp. 14964-14971,1993 0 1993 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in U.S.A. Insulin-like Growth Factor(1GF)-independent Action of IGF-binding Protein-3 in Hs578THuman Breast Cancer Cells CELL SURFACEBINDINGANDGROWTHINHIBITION* (Received for publication, February 16, 1993,and in revised form, March 17, 1993) Youngman Oh$, Hermann L. Muller, George Lamson,and Ron G. Rosenfeld From the Dewartment of Pediatrics. Division of Pediatric Endocrinology, Stanford University Schoolof Medicine, ~ - I ~~ ~ Stanford, Caiifornia 94i05 Estrogen receptor-negative Hs578T human breast Insulin-like growth factors, IGF-I andIGF-11,’ are peptide cancer cells secrete insulin-like growth factor binding mitogens for multiplecell lines, including human breast can- protein (1GFBP)-3and IGFBP-4as major binding pro- cer cells (1-3). They share structural similarity with insulin tein (BP) species. Our previous immunohistochemical and have their own high affinity receptors on the cell mem- studies (Oh, Y.,Muller, H. L., Pham, H., Lamson, G., brane (4). Themitogenic actions of both IGF-I and-11 appear and Rosenfeld, R. G. (1992) EndocrinoEogy 131, to be mediated through the type 1IGF receptor, which has 3123-3125) have