Applied Microbiology June 2009英文电子书.pdf

Journal of Applied Microbiology ISSN 1364-5072 ORIGINAL ARTICLE Lethal effect of chitosan-Ag (I) films on Staphylococcus aureus as evaluated by electron microscopy ´ 1 ´ 2 ´ 1 J. Dıaz-Visurraga , A. Garcıa and G. Cardenas 1 CIPA-Chile, Faculty of Chemistry Sciences, Department of Polymers, Advanced Materials Laboratory, University of Concepcion, Concepcion, Chile ´ 2 Faculty of Biological Sciences, Department of Microbiology, University of Concepcion, Concepcion, Chile Keywords Abstract cell lysis, chitosan-Ag (I) film, electron microscopy, filamentation, lethal effect, Aim: This article investigated the lethal effect and morphological changes on Staphylococcus aureus . Staphylococcus aureus strains ATCC 25923 and ATCC 6538P produced by chitosan-Ag (I) films as observed by electron microscopy. Correspondence Methods and Results: The antimicrobial activity of films against staphylococci ´ Galo Cardenas, CIPA-Chile, Faculty of was determined using the broth dilution method and agar diffusion test. Kill- Chemistry Sciences, Department of Polymers, ing curves, transmission and scanning electron microscopy (TEM and SEM) Advanced Materials Laboratory, University of Concepcion, Edmundo Larenas 129 Barrio techniques were employed to evaluate the bacterial death and morphological