探讨病理性瘢痕中结缔组织生长因子的免疫电镜观察.docVIP

精品论文 参考文献 探讨病理性瘢痕中结缔组织生长因子的免疫电镜观察 大庆油田总医院 163000 　　摘要：目的：探讨结缔组织生长因子（CTGF）在病理性瘢痕组织中的表达定位，以探讨其在病理性瘢痕中的作用及与病理性瘢痕形成的关系。方法：分别留取正常皮肤2例、浅表性瘢痕3例、增生性瘢痕、增生性瘢痕边缘正常皮肤、瘢痕疙瘩及瘢痕疙瘩边缘正常皮肤各5例组织标本，用特异性抗体作为标记物，用胶体金作示踪物进行免疫电镜观察，观察标记物所在位置，以了解在不同组织中表达水平的差异性。结果：CTGF在增生性瘢痕、瘢痕疙瘩、瘢痕疙瘩边缘正常皮肤中的表达均明显高于正常皮肤、浅表性瘢痕及增生性瘢痕边缘正常皮肤。免疫电镜标记显示成纤维细胞细胞超微结构清晰，金颗粒呈团状、点灶状分布，特异性强，CTGF蛋白阳性标记主要位于粗面内质网，粗面内质网核糖体、胶原纤维、细胞外基质、桥粒连接、常染色质等部位也有表达。结论：CTGF与病理性瘢痕的形成有相关性，在其发病过程中可能发挥重要作用。 　　关键词：结缔组织生长因子（CTGF）：病理性瘢痕；免疫电镜；胶体金 　　[Abstract]Objective：To investigate the expression and localization of connective tissue growth factor（CTGF）in pathological scar tissue，and to explore its role in pathological scar and its relationship with the formation of pathological scar.Methods：We collected 2 cases of normal skin，superficial scar，3 cases of hypertrophic scar，hypertrophic scar and keloid and normal skin edge edge of keloid and normal skin tissue samples of 5 cases each，with specific antibodies as markers of immune electron microscopy using colloidal gold as a tracer，observation marker the location，in order to understand the differences in expression levels in different tissues.Results：the expression of CTGF in hypertrophic scar，scar and normal skin was significantly higher than that in normal skin，superficial scar and normal skin.Immunoelectron microscopic markers showed fibroblast cell ultrastructure clear，gold particles were spotty group，specificity，CTGF protein positive staining was mainly located in the endoplasmic reticulum，endoplasmic reticulum ribosomes，collagen and extracellular matrix，desmosomes，chromatin and other parts also have the expression.Conclusion：there is a correlation between CTGF and pathological scar formation，which may play an important role in the pathogenesis of the disease. 　　[Key words]connective tissue growth factor（CTGF）：pathological scar；immuno electron microscope；colloidal gold 　　临床上常见的病理性瘢痕包括增生性瘢痕和瘢痕疙瘩，是皮肤对创伤、炎症、烧伤应激的一种过度的皮肤纤维增生性疾病，其形成原因至今尚未完全探明。随着研究的深入，人们渐渐认识到结缔组织生长因子（connective tissue growth fac－tor，C