探讨蛋白酶体功能障碍对散发性帕金森病发病机制的影响.docVIP

精品论文 参考文献 探讨蛋白酶体功能障碍对散发性帕金森病发病机制的影响 （广东三九脑科医院神经内一科 广东 广州 510510） 【摘 要】目的：探讨蛋白酶体功能障碍对散发性帕金森疾病的发病机制研究。方法：将蛋白酶抑制剂注入大鼠的左侧黑质致密部位，构建蛋白酶体功能障碍模型作为观察组，对照组采用生理盐水注射，观察两组大鼠的行为学改变情况，通过HE染色检测路易（小）体形成率，RT-PCR、Western blotting分子生物学方法检测黑质部位alpha;-突触核蛋白（alpha;-Syn）表达情况。结果：HE染色结果均显示路易（小）体形成率观察组高于对照组，两组间相比具有统计学差异（P＜0.05）；RT-PCR、Western blotting分子生物学检测结果显示alpha;-突触核蛋白观察组显著高于对照组，两组间相比具有统计学差异（P＜0.05）。结论：蛋白酶体功能障碍可以导致路易（小）体形成率及黑质部位alpha;-突触核蛋白（alpha;-Syn）形成是散发性帕金森疾病的危险因素。 【关键词】路易（小）体形成率；alpha;-突触核蛋白（alpha;-Syn）；散发性帕金森；发病机制 【中图分类号】R742 【文献标识码】A 【文章编号】1004-6194（2015）02-0330-02 To investigate the impact of proteasome dysfunction in sporadic Parkinsons disease pathogenesis 【Abstract】Objective: To investigate the proteasome dysfunction in the study of the pathogenesis of sporadic Parkinsons disease. Methods: Protease inhibitor injection site in substantia nigra of rat left the building proteasome dysfunction model as the observation group, the control group with saline injection, observe the behavior of the two groups of rats changes the situation, by HE staining Louis (small) formation rate, RT-PCR, Western blotting molecular biology methods to detect the substantia nigra alpha;- synuclein (alpha;-Syn) expression. Results: HE staining showed Louis (small) formation rate were higher, compared with the significant difference between the two groups (P lt;0.05); RT-PCR, Western blotting molecular biology test results show that alpha;- synuclein observation group were significantly higher compared with the significant difference between the two groups (P lt;0.05). Conclusion: The proteasome dysfunction can lead Louis (small) formation rate and the substantia nigra alpha;- synuclein (alpha;-Syn) is a risk factor for sporadic form of Parkinsons disease. 【Keywords】Louis (small) formation rate; alpha;- synuclein (alpha;-Syn); sporadic Parkinson; Pathogenesis 原发性帕金森是一种慢性的神经系统疾病，是一种中老年的常见性疾病，临床上主要表现为震颤、肌肉强直、运动迟缓等，主要的病理特征是出现黑质的致密部位多巴胺能神经元的选择性丧失，出现残存路易小体，该病包括帕金森病痴呆和路易体痴呆，