含氟核苷在生物大分子结构机理研究中的应用进展Application of Fluoro.pdf

基因组学与应用生物学，2010 年，第29 卷，第4 期，第740-747 页 Genomics and Applied Biology, 2010, Vol.29, No.4, 740-747 评述与展望 Review and Progress 含氟核苷在生物大分子结构机理研究中的应用进展 1,2 2 1* 魏占磊 朱静 李荣东 1 湖南中医药大学药学院, 长沙, 410208; 2 中国科学院上海有机化学研究所生命有机国家重点实验室, 上海, 200032 * 通讯作者, rongdongli@ 摘 要 氟原子半径小、电负性强、具有与(-OH)官能团类似的氢键形成能力，是“超级卤素”。生物大分子 核酸中引入氟原子，会使得氟原子附近的原子电子云密度、碱性及酸性发生明显变化，从而导致整个核酸 分子构象由于偶极相互作用发生变化。本文主要综述最近几年含氟核苷在蛋白质与核酸的相互作用及其 动力学机制、DNA 和RNA 的二级结构等生物大分子结构机理研究中的应用，同时，进一步阐述了利用含 氟核苷，结合19 F-NMR 核磁共振波谱技术，研究核酸与蛋白质之间特异性识别的结构与动态机制及核酸 二级结构的研究进展，以期氟化学在我国化学和生物学领域得到更为广泛的应用。 关键词 氟 19 , 核酸, 蛋白质, 分子结构, 动力学, F-NMR Application of Fluoro-Contained Nucleotide in Biological Molecular Struc- tural Mechanism Wei Zhanlei 1,2 Zhu Jing 2 Li Rongdong 1* 1 College of Pharmacy Hunan University of Traditional Chinese Medicine, Changsha, 410208; 2 State Key Laboratory of Bioorganic, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032 * Corresponding author, rongdongli@ DOI: 10.3969/gab.029.000740 Abstract In all the elements, fluorine has small atomic radius, strongest electronegativity, and similar hydrogen bond forming ability with hydroxyl group (-OH), it is so called “super-halogen ”. The introduction of fluorine atoms to nucleic acids of biological macromolecules will change electron density of neighboring atoms, alkalinity and acidity significantly, resulting in the DNA conformation changes as the dipole-dipole interaction. In this pa- per, we mainly reviews the recent application of fluorinated nucleoside in the mechanism and structure of biolog- ical macromolecules on the interaction between protein and nucleic acid and its dynamics mechanism. The sec- ondary structure of DNA and RNA and so on. On the other hand, we further describes the recent progress of flu- orinated nucleosides in studying the specific recognition of nucleic acids and proteins