不同干预对肾小管上皮细胞connexin43的表达及白蛋白通透性的影响.doc

不同干预对肾小管上皮细胞connexin43的表达 及白蛋白通透性的影响 杨黎 甘华 杜晓刚 哈伟杰 郑开元 [摘要] 目的 观察高糖及不同浓度胰岛素作用下肾小管上皮细胞间隙连接蛋白（connexin43）的表达，以及该蛋白的表达量与白蛋白通透水平的关系。方法 用高糖及不同浓度胰岛素刺激肾小管上皮细胞，检测不同分组中connexin43蛋白的表达。用免疫荧光分析connexin43蛋白的表达；用RT-PCR检测connexin43 mRNA的表达。利用Transwell小室检测不同条件下肾小管上皮细胞对白蛋白的通透水平，并观察以上指标之间变化有无相关性。结果 高糖及不同浓度胰岛素可以使肾小管上皮细胞connexin43的表达减少，单层肾小管上皮细胞通透性升高;而高糖刺激肾小管上皮细胞后，加入不同浓度胰岛素干预，connexin43的表达增加，单层肾小管上皮细胞通透性下降。讨论 肾小管上皮细胞connexin43蛋白的表达与细胞对白蛋白的通透性相关。 [关键词] 连接蛋白43 上皮细胞 肾小管 白蛋白 通透性 The expression of connexin43 protein and the permeability of albumin on HKC which was stimulated with high glucose and insulin Yang Li Gan Hua Du Xiao-gang Ha Wei-jie Zheng Kai-yuan Department of Nephrology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China Corresponding author: Gan hua ,Email：zxg0233@ [Abstract] Objective To observa the expression of gap junction protein (connexin43) and the permeability of albumin of cultured human renal tubular epithelial cells(HKC) which was stimulated with high glucose and insulin,and the relationship between the expression of connexin43 and the change of permeability of HKC METHOD The normal HKC was stimulated with high glucose and insulin, to detective the expression of connexin43 protein of HKC in different groups. The expression of connexin43 protein and mRNA was detected by Immunity fluorescence and RT-PCR．The the permeability of albumin in different groups was examinated with Transwell cab,and observes whether there is relevance between above datas. Result Insulin and high glucose stimulated HKC ,down-regulated the connexin43 protein expression ,and up-regulated the cell permeability. The high glucose stimulated the HKC, then add different concentrations of insulin, the expression of connexin43 increased,the permeability of single-layer tubular epithelial cells decreased Conclusion The expression of connexin43 protein of HKC and the cell permeability is relevant.