甲基叔丁基醚共代谢基质研讨.pdf

甲基叔丁基醚共代谢基质研究 1,2 1,2 1,2 1,2 1,2* 张瑞玲 ，欧志龙 ，黄国强 ，姜斌 ，李鑫钢 （1.天津大学化工学院，天津300072； 2.精馏技术国家工程研究中心，天津300072） 摘要： 甲基叔丁基醚（MTBE）污染已经成为国外地下水环境污染中比较关注的问题。在我国的研究则刚刚起 步。本文筛选得到一株可以以MTBE为唯一碳源生长的菌株，为了更好的提高其生物积累量并提高其降解活 性，探讨了添加共代谢基质对其降解的影响，分别以容易被利用的葡萄糖，乙醇，丙三醇为研究对象。结果 表明，降解体系中添加丙三醇能够起到很好的促进效果，并且降解周期也大大缩短。随后对共代谢基质丙三 醇的浓度进行了优化，研究结果表明，当丙三醇与MTBE的比例为1：1时，6天内降解效果达到80.2%。该研究 为MTBE的生物修复提供了新的思路。 关键词： 甲基叔丁基醚； 共代谢；生物降解 Study on Cometabolic of MTBE ZHANG Rui-ling1,2, OU Zhi-long1,2, HUANG Guo-qiang1,2 , JIANG Bin1,2 , LI Xin-gang1,2 (1. School of Chemical Engineering and Technology of Tianjin University; Tianjin 300072, China; 2. National Engineering Research Centre for Distillation Technology, Tianjin 300072, China) Abstract ：The contamination of groundwater by methyl tert-butyl ether (MTBE) has become widespread. As a result, the bioremediation of MTBE is becoming an active area of research, but in China, there are very few reports about the situation of the pollution of MTBE and the remediation technology. In this study, a MTBE degrader was isolated from MTBE polluted soil. And the effect of cometabolic substrates on degradation of MTBE for increasing the biomass and promoting the biodegradation ability were investigated. The substrates included glucose, ethanol and glycerol. The result showed, glycerol was the best cometabolic substrate, which could effectively promote the degradation rate and shorten the period significantly. Further more, the 收稿日期：2006-08-20 基金项目：国家自然科学基金（No.）；天津市自然科学基金（06YFJMJC06800 ） 作者简介：张瑞玲，女，1979~，博士研究生，主要研究方向为土壤与地下水的生物修复研究， Em