百合杂交后代的RAPD分析研究.pdf

百合杂交后代的RAPD分析木 张克1赵祥云1王文和1路苹1薛敦孟2耿哲1 (1．北京农学院园林系102206；2。北京润博球根施卉有限公词100029) 殛进褥抢救获褥杂交后代，并将杂交艨代进行继代组织培养。提取亲本及杂交后代的总DNA，露选用不同弓l物，秘用 RAPD技术对紊本及杂交蔚代进行分析，判断杂交詹代成功与否。研究不闭条件对RAPD效果的影响，寻求最佳的分析 方案。初步研究结果：选用的五种引物都能扩增出条带，比较亲本和其杂交后代的带谱看出：山丹与白天使的杂交后代 骞霹能蠹龚杂交；薅淫丹毒冬天夔的杂交蘑代不能刿鼗是否势粪杂交。 关键词百合；继代培养；RAPD分析 RAPD of Lily analyzeshybridprogenies Xue Zhel KelZhao Wen_he‘Lu Zhang XiangyunlWang Pin91Dunmen92Geng RainbowFlowerBulb 《1．BeijingAgriculturalCollege，Beijing，102206；2．Beijingco。，Ltd。，Beijing，100029) of Lcollco]orבnavonawereusedinthis Abstract弧e Ialium hybridprogenies pumilumבnavona’and didsubcultureonthe extractedtheDNAofthemandtheir chose study．We hybridprogenies，then parents．We if wag RAPD to differentrandom tO thosematerialsandconcludedthatit succegsfRl enlarge by analyzing primer the the8anlewe the ofdifferentconditionstotheeffectofRAPD timestudied enlargedproduct．At impact analyze． Theresultswereasfollows：thereWagall Call outdifferentDNA inthe and primerenlarge string hybridprogenies andtheother their hy妊d of successful hybridprogenies parents．ThepmgeniesL．pumilumדilgvona”wag was鞋l措珏eeess{堪bytheDNA of comparingstringsamples． Keywords LzTy；subculture；RAPDanalyze+ 远缘杂交是培