Lawsonia intracellularis in a dog with inflammatory ….pdfVIP

Vet. Med. – Czech, 48, 2003 (5): 141–145 Original Paper Lawsonia intracellularis in a dog with inflammatory bowel disease 1 5 2 2 3 4 6 R. H����� , J. K����� , K. T������� , J. S���� , R. H������� , F. T���� , J. B������ 1 2 Small Animal Clinic, Faculty of Veterinary Medicine, Department of Microbiology and 3 Immunology, Faculty of Veterinary Medicine, Department of Pathologic Morphology, 4 Faculty of Veterinary Medicine, Department of Anatomy, Histology and Embryology, 5 Faculty of Veterinary Medicine, Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 6Institute of Pathologic Anatomy, Faculty Hospital Bohunice, Brno, Czech Republic ABSTRACT: A two-year-old male German short-haired pointer was presented with a 1.5-year history of intermit- tent small-bowel diarrhoea. Inflammatory bowel disease (chronic lymphocytic-plasmacytic gastritis, enteritis and colitis) was diagnosed on the basis of histological examination of biopsies obtained on repeated endoscopy and by exclusion of other possible causes. Warthin-Starry silver staining of stomach mucosa revealed the presence of gastric spiral organisms. The evidence of L. intracellularis was provided by a positive nested polymerase chain reaction in one biopsy of duodenal mucosa and in one rectal smear. In 5 blood sera collected over a period of 8 months the IgG antibodies to L. intracellularis were found by an indirect fluoresc