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中国农学通报 2017,33(3): 15-18 Chinese Agricultural Science Bulletin 人工老化对小麦种子活力及超弱发光的影响 田 茜，李 群，段乃彬，颜廷进，戴 双，王 栋，张文兰 （山东省农作物种质资源中心，济南250100 ） 摘 要：为阐明人工老化对种子超弱发光(UWL)的影响及UWL 与种子活力的相关性，探索一种种子活 力无损检测新方法，以小麦种子‘济麦22 ’为材料，研究了人工老化过程中超弱发光的变化及其与种子 活力、ATP 含量的关系。研究结果显示：随着老化时间的延长，种子的发芽率、发芽势、发芽指数、UWL 强度和ATP 含量均逐渐下降；人工老化48 h 后，与对照相比分别降低了19.19% 、34.34% 、38.78% 、27.77% 和13.84% 。相关分析表明，UWL 强度与发芽率、发芽势、发芽指数和ATP 含量显著正相关。因此UWL 作为一种快速、无损、灵敏的检测方法可应用于种子活力无损检测研究。 关键词：小麦；人工老化；种子活力；超弱发光；ATP 含量 中图分类号：S330.3+ 1 文献标志码：A 论文编号：cas EffectsofArtificialAgingonSeedVigorandUltraweakLuminescenceofWheatSeeds TianQian,LiQun,DuanNaibin,YanTingjin,DaiShuang,WangDong,ZhangWenlan (ShandongCropGermplasmResourcesCenter,Jinan250100) Abstract: In order to ascertain the effect of artificial aging on ultraweak luminescence (UWL) and the correlationbetweenUWLandseedvigor,andexploreanewwayofseedvigornon-destructivetesting,seedsof wheatvariety ‘Jimai22’wereusedtostudythechangeofUWLintensityanditsrelationshiptoseedvigorand ATPcontentduringartificialaging.Theresultsshowedthatwiththeextensionofartificialagingtime,the germinationpercentage,germinationpotential,germinationindex,UWLintensityandATPcontentdecreased gradually;afterartificialagingfor48h,theywerereducedby 19.19%,34.34%,38.78%,27.77%and 13.84%, respectively, compared with CK. Correlation analysis indicated that the UWL intensity was significantly positivecorrelatedwithgerminationpercentage,germinationpotential,germinationindexandATPcontent.It wasconcludedthatUWLasafast,non-destructiveandsensitivedetectionmethodcouldbeappliedinthe studyofseedvigornon-destructivetesting. Keywords:wheat;artificialaging;seedvigor;ultraweakluminescence;ATPcontent 0 引言