副溶血性弧菌长期保存方法探讨-环凯微生物科技.pdf

副溶血性弧菌长期保存方法探讨 1 1 1 1 2 1 李艳嫦 , 蔡芷荷 , 卢勉飞 , 田亮 , 吴清平 , 李兴 (1. 510663) (2. 510070) 摘要：目的：针对实验室采用低温冻存法不能长期保存副溶血性弧菌的问题，探寻适合副溶血性弧菌( Vibrio parahemolyticus )长期稳定保存的简易方法。方法：采用 3.5%氯化钠半固体室温保存法保存副溶血性弧菌， 定期活化，观察生长情况，并进行生化确认。结果：副溶血性弧菌采用 3.5%氯化钠半固体加液体石蜡密封 室温保存法保存期达 24 个月以上，且基本生物学特性均保持稳定。结论：此法操作简便，适宜副溶血性弧 菌的长期保存且生物学特性稳定。 关键词：副溶血性弧菌 长期保存 稳定 Study on the method of long-term preservation for Vibrio parahemolyticus 1 1 1 2 2 1 LI Yan-chang ,CAI Zhi-he , LU Mian-fei ,TING Liang , WU Qing-ping , LI Xing (1. Guangdong Huankai Microbial Sci. Tech. Co., ltd., Guangzhou, Guangdong 510070, China) (2. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China) ABSTRACT OBJECTIVE: To study the method of long-term preservation for Vibrio parahemolyticus for overcome the difficult of long-term preservation under low temperature. METHODS: V. parahemolyticus were preserved in 3.5% NaCl Semi-solid Medium at room temperature, revived in fixed period, observed growth, and performed biochemical identification . RESULTS V. parahemolyticus were preservated in 3.5% NaCl Semi-solid Medium,covered with liquid paraffin, at room temperature for over 24 months, and grew well in 3% NaCl Tryptose Soya Agar while revived from storage status, biological characteristics remained stable .CONCLUSIONS This method is suitable for V. parahemolyticus preservation in a long-term time, and the biological characteristics stability. KEY WORDS ：V. parahemolyticus; preservation; stability 副溶血性弧菌(Vibrio parahemolyticus)是海洋及盐湖极为广泛的一种致病性嗜盐菌，若 作者简介：李艳嫦， 女，硕士，助理工程师，主要从事微生物快速检测技术的研究工作，E-mail