伏核内勿动蛋白a和降钙素基因相关肽1受体在炎性痛大鼠痛觉调制.pdf

2013年，第28卷，第1期 ·基础研究· 伏核内勿动蛋白A 和降钙素基因相关肽1受体 在炎性痛大鼠痛觉调制过程中的角色* 房春燕 王 燕 王亚南 孟 蒂 张广学 李 宁1 1 1 1 1 1,2 摘要 目的：探讨大鼠伏核内勿动蛋白A（Nogo-A）和降钙素基因相关肽1（CGRP1）受体在痛觉调制过程中的作用。 方法：健康雄性SD大鼠随机分为4组：空白对照组，伏核内注射生理盐水1μl；炎性痛模型对照组、吗啡组和纳洛酮 组。福尔马林致炎成功后，分别向其伏核内注射生理盐水、吗啡、纳洛酮各1μl。采用Western blot法，观察各组大 鼠伏核内Nogo-A和CGRP1受体蛋白表达的变化，分析炎性痛、吗啡、纳洛酮对大鼠伏核内Nogo-A和CGRP1受体蛋 白表达的影响。 结果：正常大鼠伏核内有Nogo-A和CGRP1受体蛋白表达；福尔马林致炎后Nogo-A的蛋白表达量降低（P0.05），而 CGRP1表达升高（P0.05）；与炎性痛模型对照组比较，吗啡组大鼠伏核内Nogo-A的蛋白表达量增高（P0.05）， CGRP1降低（P0.05），而纳洛酮组Nogo-A降低（P0.05），CGRP1增高（P0.05）。 结论：Nogo-A和CGRP参与了炎症致痛敏大鼠伏核内的痛觉调制过程，且二者之间存在反向调节关系，内源性阿片 系统可能参与并影响了该过程。 关键词 勿动蛋白-A；降钙素基因相关肽受体；吗啡；伏核；痛觉调制 中图分类号：R49 文献标识码：A 文章编号：1001-1242（2013）-01-0009-04 Role of neurite outgrowth inhibitor-A and calcitonin gene related peptide 1 receptor in pain modulation in nucleus accumbens of rats with inflammatory pain/FANG Chunyan, WANG Yan, WANG Yanan, et al. //Chinese Journal of Rehabilitation Medicine, 2013, 28(1): 9—12 Abstract Objective: To investigate the role of neurite outgrowth inhibitor-A(Nogo-A) and calcitonin gene related peptide 1 (CGRP1)receptorinpainmodulation innucleus accumbensofrats. Method: Healthy male SD rats were randomly divided into four groups：blank control group (saline was injected to the nucleus accumbens for 1μl); inflammatory pain model control group (formalin was injected to rats hindpaw to induce inflammatory pain); morphine group and naloxone group (after inflammatory pain models were prepared, morphine and naloxone were injected to nucleus accumbens for 1μl respectively). The Western blot technique was used for Nogo-A and CGRP1 receptor protein recording. The influence