基因,蛋白,免疫 课程之蛋白组学 2.pdf

422 Applications of yeast in biotechnology: protein production and genetic analysis Geoffrey P Lin Cereghino* and James M Cregg Improvements in yeast expression systems, coupled with the tion and amidation, as well as some types of phosphorylation development of yeast surface display and refinements in two- and glycosylation [3]. Recent findings, however, should help hybrid methodology, are expanding the role of yeasts in the to alleviate some of these problems and broaden the scope process of understanding and engineering eukaryotic proteins. of future applications for yeasts in biotechnology. Addresses Yeasts as protein factories Department of Biochemistry and Molecular Biology, Oregon Graduate Yeasts have been used since the early 1980s for the large- Institute of Science and Technology, 20 000 NW Walker Road, scale production of intracellular and extracellular proteins Beaverton, Oregon 7006-8921, USA of human, animal, and plant origin [4,5]. The expression of *e-mail: geoffc@bmb.ogi.edu Correspondence: James M Cregg; e-mail: cregg@bmb.ogi.edu a foreign protein in yeasts consists of four steps: firstly, cloning of a foreign protein-coding DNA sequence within Current Opinion in Biotechnology 1999, 10:422–427 an expression cassette containing a yeast promoter and 0958-1669/99/$ — see front matter © 1999 Elsevier Science Ltd. transcriptional termination sequences; secondly, transfor- All rights reserved. mation and stable maintenance of this DNA fusion in the Abbreviations