中国明对虾(Fenneropenaeus chinensis)血蓝蛋白基因cDNA的克隆与精选.pdf

cDNA1 12 12 12 1 12 1 1 1 (1. 266071 2. 100039) 3’5’RACE Fenneropenaeus chinensis FCHc, FCHc cDNA 2161bp2034bp678 77.59KDaFCHc Litopenaeus vannamei 82% Marsupenaeus japonicas 85%Realtime-PCR FCHc WSSV FCHc cDNA cloning and sequence analysis of hemocyanin in Fenneropenaeus chinensis SUN Jie 1, 2 WANG Bao-jie 1 SUN Shu-juan 1, 2 LI Xiao-hua1 JIANG Shan 1, 2 LIU Mei 1 JIANG Ke-yong 1 Wang Lei 1 1 ( Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071) 2 ( Graduate School of Chinese Academy of Sciences, Beijing 100039) ABSTRCT A hemocyanin gene (FCHc) was cloned from hepatopancreas of Chinese shrimp Fenneropenaeus chinensis by Rapid Amplification Complementary DNA Ends (RACE) method. The full length cDNA of FCHc consists of 2,161bp with a 2,034 bp Open Reading Frame (ORF)which encoded 678 amino acidsand the predicted molecular mass was 77.59kDa. It showed 82% and 85% homology with those of Litopenaeus vannamei and Marsupenaeus japonicas . The real-time PCR results showed that the expression level of FCHc in hepatopancreas was the highest among seven studied tissuesReal-time PCR analysis showed that the expression of FCHc was up-regulated distinctly in the hepatopanereas of shrimp when they were challenged by Vibrio anguillarum and white spot syndrome virus (WSSV). FCHc showed different expression profiles in the hepatopancreas during V. anguillarum or WSSV virus infection The results implied that FCHc might play an important role in the shrimp immune system KEY WORDS Fenneropenaeus chinensis Hemocyanin Gene cloning Seque