20113等4个茶树新品系亲缘关系的est-ssr分析.pdfVIP

6 5 Vol. 6 No. 5 2015 5 Journal of Food Safety and Quality May. , 2015 2011-15-3 4 EST-SSR 雷 雨, 段继华, 罗 意, 黄飞毅, 董丽娟, 李赛君* ( , 410125) 摘 要: 目的 2011-15-3 4 , 方法 24 EST-SSR , 4 7 PCR , UPGMA , 结果 24 69 , 2.88; (Ho)(He)Shannon 0.420.540.75, 0.47~0.890.840.790.790.78 UPGMA , 0.66 7 3 : 2011-34-1, Ⅱ2011-21-1 2011-21-3, Ⅲ2011-15-3结论 4 关键词: ; ; EST-SSR Analysis of genetic relationship of 2011-15-3 and other 3 new strains of tea based on EST-SSR markers * LEI Yu, DUAN Ji-Hua, LUO Yi, HUANG Fei-Yi, DONG Li-Juan, LI Sai-Jun (Hunan Tea Research Institute , Changsha 410125, China) ABSTRACT: Objective To analyze the genetic relationship of 2011-15-3 and other 3 new strains of tea, so as to offer some information for the new strains identification, new varieties registration and parental selection. Methods The 7 tea accessions were analyzed by the 24 pairs of EST SSR primers derived according to references. Based on EST-SSR data, a dendrogram was constructed by UPGMA method. Results 69 alleles were amplified totally using 24 EST-SSR primers, on average of 2.88. The observed heterozygosity (Ho), the expected heterozygosity (He) and the Shannon index were 0.42, 0.54 and 0.75 on average, respectively. The similarity coefficient varied from 0.47 to 0.89. The similarity coefficient offspring of Zhuyeqi, Bixiangzao and Gaoyaqi