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四种新型合成的CpG寡脱氧核苷酸对淡水长脚 大虾的免疫调节特性
Open Journal of Fisheries Research 水产研究, 2017, 4(4), 166-179
Published Online December 2017 in Hans. /journal/ojfr
/10.12677/ojfr.2017.44024
Immunoregulatory Characteristics of Four
Novel Synthetic CpG Oligodeoxynucleotides
for Freshwater Giant Prawn
(Macrobrachium rosenbergii)
*
Hung-Hung Sung , Yin-Han Yang, Wei-Xuan Lai
Department of Microbiology, Soochow University, Taipei Taiwan
th th th
Received: Dec. 4 , 2017; accepted: Dec. 19 , 2017; published: Dec. 29 , 2017
Abstract
Different sequences of CpG oligodeoxynucleotides (CpG-ODNs) have been demonstrated to result
in different expressions of prophenoloxidase gene (propo) in freshwater giant prawn (Macrobra-
chium rosenbergii). In this study, in order to assess the possible applications of CpG-ODNs with
different immunomodulatory effects that may be used in aquaculture, four novel CpG-ODNs
(ODN3006, ODN4006, ODN2050 and ODN2051), which have been designed according to the dif-
ferent sequences of CpG-ODNs that promote or inhibit human and fish immunity, are used to de-
termine their effect on prawn immunity. First, the binding rate of ODN to hemocytes analyzed by
flow cytometry showed that the lowest concentration of ODN2005 required to binding 50% he-
mocytes (BC50) was at 1.88 μg/mL and that the concentration was at 5 μg/mL of ODN2050; it could
bind more than 90% hemocytes. After hemocytes treated with different concentrations of ODN in
vitro, all four ODNs shown increase the extracellular total PO activity (POT) secreted from hemo-
cytes, OND2050 at 0.67 μg/mL which not only significantly enhanced POT (p 0.01), but also
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