中国水仙水通道蛋白基因cDNA克隆序列分析与表达-福建农业学报.PDFVIP

福建农业学报26( 3) : 371~ 376, 2011 : 1008- 0384 (20 11) 03- 37 1- 06 F uj ian Journal of A g ricultural Sciences cDNA 林江波, 王伟英, 邹 晖, 戴艺民 ( , 363005) : ( SSH ) , RA CE RT-PCR , 1 N tP IP 1867 bp ( ORF) , 288 , 3068 kD, 897 Nt PIP1 : NtPIP 1 6 , 5 , A C E , BD NC , N 55 , C 9 , MIP H INPAVT FG 2 NPA ( 2b5fA) : 12 PIP1 91% , PIP1 RT-PCR : N tPIP 1 : ; ; ; : S 6822 1 : A Cloning, Sequence Analysis andExpression of cDNA Encoding Aquaporin Nt PI P 1 of Narcissus tazett a var chinensis LIN Jiang-bo, WA NG Wei- ing , ZOU H ui, DAI Y-i min ( Sugarcane Research I nstitute, F uj ian A cademy of A gricultural S ciences, Zhangz hou, Fuj ian 363005, China) Abstract: Based on constructed different florescence suppression subtractive h bridization ( SSH ) of N arcissus taz etta var chinensis, an encoding transmembrane aquaporin ( A QP) gene N tPIP 1 w as separated and cloning b RA CE and RT-P CR The NtPIP1 cDNA w as 867bp, encoding a protein of 288 amino acids w ith a predicted molecular mass of 3068kD and theoret ical isoelectric point of 897 T he Nt PIP1 exhibited a t pical structure w ith six t ransmembrane domains which linked b five loops, loop A , C and E w ere at outside of membrane, loop B, D, N terminal and C terminal w ere at inside of membrane N terminal has 55 amino acids and C terminal has 9 amino acids T he NtPIP 1 has the consensus sequence H INPAVT FG of MIP famil and tw o highl conserved peptides Asn- Pro-Ala ( NPA) The protein 3D structure w as predict ed b homolog comparat ive modeling in Sw iss-Model, the result from Swiss-Model showed that the 3D structure w as highl similitude w ith S inacia olerace