基因多态性与冠心病PPT.ppt

The concentrations of C-reactive protein in different genotype groups Gene type n CRP median Inter-quartile range CC 121 2.05 * 0.67-3.64 * CT 9 3.98 1.69-6.68 * (P0.05) The TaqⅠpolymorphism of IL-1β was associated with the concentrations of CRP in normal people The TaqⅠpolymorphism of IL-1β was not associated with with CHD CRP, The first acute-phase protein to be described, its plasma concentration increases during inflammatory states. Recently, CRP might have an important role in the pathogenesis and prediction of CHD. 1.7 CRP gene and CHD Polymorphism analysis Figure 1-1 Determination of the +1444 C/T polymorphism in the CRP gene by PCR-RFLP. Lane M：DNA Marker; Lane A：PCR product; Lane B：homozygous CC; Lane C：heterozygous CT; Polymorphism analysis Figure The chromatogram of homozygous CC Comparison of distribution of +1444 C/T genotypes and alleles between CHD group and control group Groups n Genotype Distribution(%) Allele Distribution(%) CC CT+TT C T CHD 128 114(89.1) 14(10.9) 242(94.5) 14(5.5) Control 119 107(89.9) 12(10.1) 226(95.0) 12(5.0) ?2 0.048 0.045 P 0.827 0.832 OR(95%CI) 0.913(0.404?2.063) * 0.918(0.416?2.027) △ *: genotype CC vs CT+TT；△：allele C vs T Fang Zheng Clinic Lab, Zhongnan Hospital, Wuhan University Coronary Heart Disease is still the No. 1 killer in the world. Genetics Environmental Life style The prevention of CHD is based on the control of several factors associated with a disease or clinical condition and suspected to play a pathogenetical role, defined as risk factors.The risk factors of CHD included: Age Clotting factors Sex Fibrinolytic factors Hypertension Hyperhomocysteinaemia Smoking Inflammation factors Diabetes Endothelium factor Hyperlipidaemia Nutrition factor Obesity Post-menopausal status Genetic factors Emerged CHD Risk Factors But only in 5% of hereditary CHD, the gene background was clear. In the others, each genetic factor played a minor role in occurrence and development of the disease. R