人类优生的辅助生育技术-优生.ppt

BETTER ASSISTED REPRODUCTIVE TECHNIQUE Application of Reconstructed Oocyte in ART Reconstructed Oocyte Why ？   Oocyte aging with advancing age！ Cytoplasm aging Accumulation of free radical and ROS Mitochondria DNA mutation ATP production↓ Nuclear aging Abnormal meiotic apparatus-Spindle Aneuploidy↑ Cytoplasm aging-Key point Role of Mitochondria in Cytoplasm Aging Energy factory mtDNA: maternal inherited, circular, histone-free of 16.6 kb of DNA. Coding 13 protein subunits required for oxidative phosphorylation. mtDNA Mutation mtDNA mutation ＜40 1/9 （11%） 40~50 9/19 （47%） ≥50 10/11（90%） ——Kitagawa T et al，Biol Reprod 1993 mtDNA deletion rate higher in advanced age women. ——Keefe DL et al， Fertil Steril 1995 Ooplasm Transfer improve oocyte quality due to mitochondria defect Injection of mitochondria can prevent oocyte from apoptosis in mice. ——Perez GI et al， Nature 2000 Ooplasm transfer helpful for aged women repeat reproductive fail due to impaired embryo development. ——Cohen J et al，Lancet 1997 Cohen ooplasm transfer 23 cycles, 12 clinical pregnancy, 8 delivery, 1 abortion. —— Brenner CA et al， Fertil Steril 2000 Abnormal spindle cause increased aneuploidy. Nondisjunction oocyte chromosome and aneuploidy rate higher in ≥40 years than that in ＜35 years（p＜0.001）. ——Dailey T et al，Am J Hum Genet 1996 25~34 35~39 40~45 Aneuploi