肺癌沉默基因.ppt

3.2。稳定转染表达pGPU/GFP/Neo/livin与pGPU/GFP/Neo/Control的特征 我们建立了SGC-7901 与任一pGPU/GFP/Neo/livin稳定转染，pGPU/GFP/Neo /Control 质粒，或空pGPU/GFP/Neo/vector (图3)。用西方吸干技术和逆转录聚合酶链反应技术选择每个转染的克隆并分析决定livin mRNA,和蛋白质表达。 其他的都被选择作为扩展和另外的研究。(如图4及5)显示， livin mRNA和蛋白质的水平在SGC-7901pGPU/GFP/Neo/livin2中转染降低了90%以上。Livin表达抑制在pGPU/GFP/Neo/livin1转染和消极控制中没有被发现。所以SGC-7901 pGPU/GFP/Neo/livin2被用来做后续实验。 Expression of livin in gastric cancer and induction of apoptosis in SGC-7901 cells by shRNA-mediated silencing of livin gene Report person ： 王晓磊 Student id ：2014050448 介导的shRNA能抑制肺癌细胞中livin沉默基因的表达从而促进SGC-7901细胞凋亡 Background Because of increased resistance to apoptosis in tumor cells, inhibition of specific antiapoptotic factors may provide a rational approach for the development of novel therapeutic strategies.Livin, a novel inhibitor of apoptosis protein family, has been found to be expressed in various malignancies and is suggested to have poorly prognostic significance. However, no data are available concerning the significance of livin in gastric cancer. In this study, we detected the expression of livin in human gastric carcinoma and investigated the apoptotic susceptibility of SGC-7901 cell by shRNAmediated silencing of the livin gene. 背景 由于肿瘤细胞抑制凋亡增殖,特定凋亡的抑制因素会对于发展新的治疗策略提供一个合理途径。Livin是一种凋亡抑制蛋白家族成员，在多种恶性肿瘤的表达中具有意义。但是, 在有关胃癌方面没有可利用的数据。在本研究中,我们发现livin基因在人类胃癌中的表达并调查了介导的shRNA能抑制肺癌细胞中livin沉默基因的表达，从而促进SGC-7901细胞凋亡。 1. Introduction Gastric cancer is one of the most common malignancies in the world. Most patients with this disease are diagnosed in advanced stages, and lose the chance of surgical eradication. Despite much progress in chemotherapy, the overall survival of the patients with gastric cancer in advanced stage is still poor. Resistance of cancer cells to chemoagents may contribute to failure of the treatment. Among the reasons of drug resistance, inhibited process of cell apoptosis may play an important role. 1．介绍 胃癌是世界上最常见的恶性肿瘤之一。大多数患者被诊断为这个疾病的阶段,在最佳时间的机会错失了手术治愈。尽管有很大改善,但处于晚期胃癌的化疗患者的总体存活率仍