乳酸菌ATCC367菌株在不同培养条件下的生长特性差异.PDFVIP

乳酸菌 ATCC367 菌株在不同培养条件下的生长特性差异 ———钟 　凯 　何庆华 　吴永宁 — 225 — 论著 乳酸菌ATCC367 菌株在不同培养条件下的生长特性差异 钟 　凯 　何庆华 　吴永宁 ( 中国疾病预防控制中心营养与食品安全所 ,北京 　10002 1) 摘 　要 : 目的　通过分析乳酸菌ATCC367 在不同p H 环境下的生长曲线和p H 值曲线的差异 ,探讨乳酸菌的生长规 律 。方法 　配制不同成分 、不同p H 值的培养基 ,分别进行菌株培养并测定 A 600 和 p H 值 ,获得生长曲线和 p H 变化 曲线 。结果 　乳酸菌 ATCC367 菌株在 p H 35 和添加壳寡糖的培养基上生长受到抑制 ,适宜生长的p H 值环境为 35～65 ,添加葡萄糖能促进其指数生长 ,添加 30 gL 的氨基酸可能不利于生长 。结论 　ATCC367 菌株可能根据生 长环境和能量来源的不同 ,动态调节不同代谢途径 , 以获得最佳生长状态 。 关键词 :乳杆菌属 ;氢离子浓度 ;培养基 ;生长曲线 Characteristics of Lactobacillus Brevis ( ATCC367) in Different Media ZHON G Kai , HE Qinghua , WU Yongning (National Institute for Nutrition and Food Safty , Chinese CDC , Beij ing 10002 1 , China) Abstract : Objective 　Rown patterns of ATCC367 strain under the conditions of six different media were discussed according to growth curves and p H curves. Methods 　six media with different ingredients or p H value were prepared and ATCC367 strain cultures were harvested from each medium. Culture samples were collected and A 600 and p H values were determined thereafter . Growth curves and p H curves were constructed according to these data . Results 　Growth curves were different between ATCC367 strain cultures. In MRS + aa p H 35 and MRS + aa + COS p H 65 media , strain growth were suppressed. p H 35～65 was suitable for its growth , glucose could extend its log phase while high concentration of amino acid maybe harmful . Conclusions 　 ATCC367 strain may have the ability of dynamic metabolic regulation to accommodate different culture environment or energy source . Key words : Lactobacillus ; HydrogenTon Concentration ; Culture Media ; Growth Curver