学术报告 英文 内容：寡糖的分离和结构鉴定.ppt

L/O/G/O L/O/G/O L/O/G/O Structural confirmation of oligosaccharides newly isolated from suger beet molasses Outlines Background Achievement Experimental Conclusion Discussion Background Why should we do this experi-ment? raffinose;other oligosaccharides;examined in detail What contributions could the study bring? new oligosaccharides;structure comfirmed;full assignment of the 1H,13C signals;2D-NMR Four oligosaccharides were isolated The structural of them were confirmed Achievement 1st The first full assignment of 1H ，13C and 2D-NMR of saccharide 1~4 were carried out 2nd Experimental Diluted five times with water MOLASSES Preparative HPLC ODS column 35℃ water 3.5mL/min 60 times DILUTED MOLASSES Measured with a Refractive Index Detector Concentrated in vacuo then freeze-dried ELUTED SACCHARIDES POWDERED FRACTIONS I to XII Experimental by HPAEC and TOF-MS analyses Fractions I to XII Fractions XI (13.8 mg) Fractions IV (51.6 mg) Fractions IX (240.0 mg) FractionsII ( 41.1 mg) Experimental carbon-Celite column 10% ethanol (1.8 –2.4 L) Fraction Fraction IV IX Fraction II SACCHARIDE1 carbon-Celite column 5% ethanol (0.3-1.1L) carbon-Celite column 10% ethanol (2.5–2.9L) SACCHARIDE2 SACCHARIDE3 Preparative HPLC repeated 60 times rentention time 34.5-35.5 Fraction XI SACCHARIDE4 Experimental 1st 2nd 3rd HPAEC Saccharide1~4 were shown to be homogene-ous,but didnt correspond to any authentic saccharides TOF-MS The polymerization was established as 3 Acid Hydrolyzates→HPLC Saccharides 12:Glucose,Fructose,Galactose(1:1:1) 34:Glucose,Fructose(2:1) Methylation and methanolysis →GLC Methylation was carried out by Hakomori method Methanolyzed by heating at 96℃ for 30min with 1.5% methanolic hydrochloric acid result saccharide 3 1-Kestose 1.10 1.32 1-Kestose 1.47 1.06 Raffinose 2.63 3.74 1.45 3.68 1.29 1.08 2.55 Methyl-1,3,4,6- tetra-O-methyl- D-fructoside Methyl-2,3,4,6- tetra-O-methyl- D-glucoside Methyl-2,3,4- tri-O-methyl-