hbd-2在大疱性类天疱疮皮损中表达的分析word论文.docxVIP

HBD-2 在大疱性类天疱疮皮损中表达的研究摘要目的：通过研究 HBD-2 在大疱性类天疱疮患者皮损组织中的表达水平及其与正常皮肤 及银屑病患者皮肤组织中表达含量的比较，探讨 HBD-2 在大疱性类天疱疮发病中的 作用。方法：选取大疱性类天疱疮（BP）患者皮损组织，寻常性银屑病患者皮损组织，健康 人皮肤组织各 20 例，用免疫组化法检测 HBD-2 在三种疾病皮损中的表达水平。其中 20 例寻常性银屑病皮损组织作为阳性对照组，20 例健康人皮肤组织作为阴性对照组。 选择平均灰度值作为参考数据，采用 SPSS18.0 统计软件进行数据分析，计量资料采 用 t 检验，组间对照选用 Wilcoxon 秩和检验，并进行分析。结果：HBD-2 的表达：正常皮肤组织的免疫组化染色 HBD-2 在基底层可见少量表达,BP 患者皮损的表皮基底层、棘层、颗粒层可见表达,银屑病患者皮损可在表皮基底层、 棘层、颗粒层可见 HBD-2 的表达。HBD-2 在 BP 表皮中的表达，高于阴性对照组正 常皮肤（Z=-10.892,P0.001），高于阳性对照组寻常型银屑病皮损部位（Z=-5.385,P0.001）。结论：HBD-2 在大疱性类天疱疮患者皮损部位的高表达推测 HBD-2 参与了大疱性类天 疱疮的发病过程；HBD-2 的高表达可以降低感染的发生，提示临床上可以根据大疱 性类天疱疮患者皮损部位的表皮脱落程度，指导抗感染治疗。关键词：大疱性类天疱疮； 防御素-2； 银屑病；Study of the Expression of β-defensin-2 in bullous pemphigoid AbstractObjective:By contras the differente expression of HBD - 2 in bullous pemphigoid ，psoriasis and the healthy people ， to explore the connection of HBD - 2 and bullous pemphigoid in pathogenesis and prognosis.Methods:Select 20 cases of bullous pemphigoid (BP) skin, 20 cases of psoriasis skin, 20 cases of healthy skin to tissue, we use immunohistochemical method to detect HBD - 2 respectively expression level in three kinds of specimens of paraffin section. 20 cases of unusual psoriasis detected as positive control group. 20 cases of healthy skin tissue as the negative control group. Choose the average grey value as reference data. Using SPSS18.0 statistical software for data analysis. The data are analyzed by t Test to analysis . Contrast between groups choose rank and inspection to analysis.Results:Beta defensive element to express: normal skin tissues of immunohistochemical staining HBD - 2 in the base layer is a small amount of expression, BP was detected in patients with full-thickness visible expression, immunohistochemical in epidermis of patients with psoriasis skin full-thickness see HBD - 2 expression.HBD - 2 expression in the BP epidermis is higher than normal skin (Z = 10.892, P 0.001), higher than detected (Z = 5.385, P 0.001).Conclusion:The high expression of HB