hsa-mir-100对人膀胱癌t24细胞增殖、侵袭以及凋亡的影响word论文.docxVIP

hsa-mir-100对人膀胱癌t24细胞增殖、侵袭以及凋亡的影响word论文.docx

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hsa-mir-100对人膀胱癌t24细胞增殖、侵袭以及凋亡的影响word论文

行为变异。关键词:hsa-miR-100膀胱癌T24细胞转染增殖侵袭凋亡2Effectofhsa-miR-100onproliferation,invasionandapoptosisofT24bladdercancercellsAbstractObjectives:Upregulatedhsa-miR-100 in bladder cancerT24 cells,we explored the influence abilityofproliferation, invasion and apoptosis.Methods:Application chemical method synthetized hsa-miR-100 mimics.TransientlytransfectedintoT24cellsandthenusedquantitativepolymerasechain reaction(Q-PCR)detectingtherelativeexpressionofhsa-miR-100inT24cells.Make surethattheT24cellshadbeensuccessfullytransfectionandonahighrate. Proliferation,invasionandapoptosisoftheT24cellswererespectivelyevaluated usingMTT experiment, transwellassayand flow cytometer(FCM) test.Results:1﹑ThebladdercancerT24cellscouldbetransfectedbyhsa-miR-100mimicsandthenusedquantitativepolymerasechainreaction(Q-PCR)detectingthe relativecontentwashigherthantheothers(P0.05).2﹑Comparedtransfected hsa-miR-100mimics group with thecontrol groupandtheblank group that thehsa-miR-100mimicsgroupcouldsignificantlyinhibitcellproliferation.(P0.05).3﹑Inthetranswellassaywecomparedtheexperimentalgroup(304.75±67.45)withthe control group(504.75±52.64)and the blank group(458.75±49.87)that thehsa-miR-100mimicsgroupcouldsignificantlyinhibitcellinvasionofthebladder cancerT24cells(P0.05).4﹑IntheFCMtestweputeachgroupintotheflow cytometricandfoundthattheapoptosisratesoftheexperimentalgroup,thecontrolgroupandtheblankgrouprespectivelywas(12.57±0.39)%,(7.71±0.31)%and(7.82±0.13)%. TheapoptosisofT24 cells obviouslyincreased (P0.05).Conclusion:Maturemimicsofhsa-miR-100couldtransientlytransfectedinto bladdercancerT24cells.Theexpressionriseofhsa-miR-100significantly suppressedproliferationforceandinvasionpowerofT24cells,andcanaccelerate T24cells apoptosis. Hsa-miR-100 May be involved in some important variation of3biological behaviour in the bladdercancer T24cells.Postgraduate: YefeiZhang( urology) Directed byProfessor: JiangboHuangKey words:hsa-miR-100,bladder cancer T24 cells,transfection,proliferation, invasio

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