microrna-429对膀胱癌细胞增殖侵袭的影响word论文.docxVIP

The Influence of MicroRNA-429 on the Proliferation and Invasion of Human Bladder Cancer CellsAbstractObjectives：To explore the effecting mechanism of miR-429 in human bladder cancer cells through observing the influence of miR-429 on the proliferation and invasion of human bladder cancer BIU-87 cells.Methods：First, differentially expressed miRNAs of normal tissue adjacent tohuman bladder cancer tissue were selected with the help of the gene chip, and their differential expressions were tested by real-time quantitative PCR. Then, a miR-429antisense nucleotide sequence（AS-miR-429）was constructed , miR-429 expressionwas down-regulated and the sequence was transiently transfected into human bladder cancer BIU-87 cells. Furthermore, the change of miR-429 expression level was detected by real-time quantitative PCR. Finally, the cell proliferation and apoptosis were detected by MTT test and flow cytometry instrument, and cell aggressivity was detected with the Trans-well test.Results：Gene chip screening found the differential expressions of miR-429,miR-143, and miR-106a in the bladder cancer tissue, including miR-429 significantly up-regulated. Real-time quantitative PCR also proved that miR-429 was significantly up-regulated in bladder cancer tissue. A miR-429 antisense nucleotide sequence was successfully constructed , and was transiently transfected into human bladder cancer BIU-87 cells. The result of transfection by real-time quantitative PCR showed that miR-429 expression decreased by 16 times in bladder cancer cells and the difference was statistically significant (P 0.05) . Forty-eight hours after transfection, MTT detection found AS-miR-429 significantly inhibited the proliferation of BIU-87 cells. Flow cytometry instrument was used to assay the apoptosis rate of BIU-87 cell, which increased significantly. While the result of Trans-well test indicated that AS-miR-429 had obvious influence on the aggressivity of BIU-87 cells.Conclusions：1.miR-429 was significantly