mir-26a在人结肠癌中的表达及其生物学功能word论文.docxVIP

miR-26a 在人结肠癌中的表达及其生物学功能研究生：李燕导师：曾希中文摘要目的：探讨miR-26a（HomosapiensmiR-26a）在人结肠癌组织的表达情况以及在结肠癌SW620细胞中的生物学功能。方法：运用qRT-PCR检测92 例结肠癌及对应癌旁正常组织中miR-26a的表达情况；采用MTT、细胞划痕实验、Transwell侵袭实验、AnnexinV-FITC/PI染色分别检测miR-26a对人结肠癌SW620 细胞的生长、侵袭和凋亡作用。结果：qRT-PCR 证实miR-26a在结肠癌组织中较癌旁组织表达显著下调，miR-26a 的表达随着结肠癌临床分期演进和浸润深度而下调，且有淋巴结转移的miR-26a 的表达显著低于无淋巴结转移组；MTT结果显示，过表达miR-26a可抑制人结肠癌SW620 细胞的生长且有时间依赖性；细胞划痕实验显示，在结肠癌SW620 细胞中转染miR-26amimics36h 后伤口愈合率为(34.7±5.5)%，对照组为(69.7±5.1) %，二者差异有统计学意义(P0.01)；Transwell侵袭实验显示，在结肠癌SW620 细胞中转染miR-26amimics36h 后穿过基底膜的细胞数为(67±14)，与对照组(154±12)比较能明显减缓细胞侵袭能力，差异有统计学意义(P 0.01)；AnnexinV-FITC/PI实验显示，转染miR-26amimics 能显著促进胃癌SW620 细胞的凋亡。结论：1.miR-26a在结肠癌组织中下调，miR-26a下调与结肠癌临床分期、浸润深度以及淋巴结转移相关。2.miR-26a可抑制结肠癌SW620细胞生长与侵袭，诱导结肠癌SW620细胞凋亡。关键词：miR-26a；结肠癌；生长与侵袭；凋亡2Theexpressionand roleofthemiR-26ainthecoloncarcinomaAbstractObjective: To analysis the expression of miR-26a in the colon carcinoma and investigatetheroleofmiR-26ain the colon carcinoma cells.Methods:TheexpressionofmiR-26awastestedbyqRT-PCR.Cellgrowthwas evaluated byMTT.Cellinvasionpotentialwasevaluated byscratchtestandtranswell assay.The apoptosis cells weretestedbyAnnexinV-FITC/PIstaining.Results:miR-26awasdownregulatedincoloncarcinomatissues.The down-regulationofmiR-26aexpressionwasassociatedwithadvancedclinicalstage andthedepthofinfiltration,andtheexpressionofmiR-26awaslowerwithlymph nodemetastasisthanwithoutlymphnodemetastasisincoloncarcinoma.miR-26a couldinhibitedgrowthandproliferationofSW620cellsinatime-dependentmanner. It’scertifiedthatmiR-26acouldinhibitcoloncarcinomacellmotilityandinvasion identified byscratch testand transwell assay.Flow cytometryanalysis determined that uponmiR-26aoverexpression, the poptosis cellsincreased significantly. Conclusions:miR-26aexpressionlevelwasdownregulatedincoloncarcinomaandhadaclose correlationtoadvancedclinicalstage,thedepthofinfiltrationandwithlymph nodemetastasis.miR-26acaninhibitcoloncarcinomacellgrowth,invasionandinducethecells apoptosis.Key words:miR-26a; colon carcinom