mir-181a、mir-181b在人胃癌细胞和组织中的表达研究word论文.docxVIP

miR-181a、miR-181b 在人胃癌细胞和组织中的表达研究硕士研究生：陈娟导师：廖爱军 教授中文摘要目的：研究 miR-181a、miR-181b 在不同分化程度人胃癌细胞株和胃癌组织中的 表达情况，探索其在胃癌发生发展过程中的作用，为胃癌的预防和诊治提供新的 理论依据。方法：体外培养 3 种不同分化程度的胃癌细胞株（AGS、SGC-7901、MGC-803） 及正常胃黏膜细胞 GES-1，收集 28 例胃癌患者手术切除的癌组织及正常组织样本， 通过 qRT-PCR 方法检测上述细胞和组织中 miR-181a、miR-181b 的表达量，比较胃 癌细胞与正常胃黏膜细胞、胃癌组织与正常胃组织中 miR-181a、miR-181b 表达的 差异性。结果：经 qRT-PCR 方法检测发现，AGS、SGC-7901、MGC-803 3 种胃癌细胞中 miR-181a 及 miR-181b 的表达量均高于 GES-1 细胞中的表达量（P0.05），而 AGS、 SGC-7901、MGC-803 3 种胃癌细胞之间 miR-181a 及 miR-181b 的表达量均无明显 差异（P0.05）。与正常胃组织相比，miR-181a、miR-181b 在胃癌组织中的表达量 显著升高（P0.05）。Ⅲ/Ⅳ期组胃癌组织中 miR-181a、miR-181b 的相对表达量高 于Ⅰ/Ⅱ期组（P0.05）。有淋巴结转移组胃癌组织中 miR-181a、miR-181b 的相对 表达量高于无淋巴结转移组（P0.05）。miR-181a、miR-181b 的相对表达量与年龄、 性别、胃癌的分化程度无明显关系（P0.05）。结论：miR-181a、miR-181b 在胃癌细胞和组织中高表达，两者的表达水平与胃 癌的分期、淋巴结转移相关，可能在胃癌的发生发展中发挥癌基因的作用。 关键词：miR-181a；miR-181b；胃癌；2Expression research of miR-181a and miR-181b in human gastric cancer cells and tissuesAbstractObjectives: The purpose of this study was to investigate the expression of miR-181a and miR-181b in different degree of differentiation of human gastric cancer cells and tissues, and to explore their role in the development and progression of gastric cancer, which provided a new theoretical basis for the prevention, diagnosis and treatment of gastric cancer.Methods: Three different degree of differentiation gastric cancer cells (AGS, SGC-7901, MGC-803) and normal gastric mucosa cells GES-1 were cultured in vitro. And 28 cases of cancerou tissues and normal tissues samples of gastric cancer patients with surgical removal were collected. Used qRT-PCR method detected the expression levels of miR-181a and miR-181b in these cells and tissues. Then compared the difference of the expressions of miR-181a and miR-181b between the gastric cancer cells and normal gastric mucosal cells, the gastric cancer tissues and normal tissues.Results：Detected by qRT-PCR method, we found that the expression levels ofmiR-181a and miR-181b in the AGS, SGC-7901 and MGC-803 three gastric cancer