mir-711在人胃癌细胞和组织中的表达及其与临床病理的相关性word论文.docxVIP

miR-711在人胃癌细胞和组织中的表达及其与临床病理的相关性研究生：贺慧鹏导师：廖爱军教授中文摘要目的:研究miR-711在多种人胃癌细胞和胃癌组织中的表达及其在胃癌发生发展中的作用，初步探讨其临床应用价值。方法:分别培养4 种不同分化程度的胃癌细胞SGC-7901、BGC -823、AGS、MGC-803、及永生化的正常胃粘膜细胞GES-1，并收集28例胃癌病人手术切除后的癌组织和正常组织，实时荧光定量PCR（qRT-PCR）检测上述细胞及组织中miR-711 的表达含量，比较其含量的差异性，并分析其与临床病理的关系。结果:经qRT-PCR对4种胃癌细胞、GES-1细胞及癌组织和正常组织中miR-711 表达含量的检测，结果显示各种胃癌细胞中的miR-711 表达含量均低于GES-1细胞的表达含量，P0.05，并表现为：GES-1SGC-7901BGC-823、AGS、MGC-803（P0.05），但在BGC-823、AGS、MGC-803胃癌细胞之间表达量无显著差异（P0.05）。相对于正常组织，miR-711 在胃癌组织中的表达量显著降低，P0.05，其表达水平与胃癌的分化程度、分期、淋巴结转移显著相关，P0.05,与年龄、性别、肿瘤大小无明显关系（P0.05）。结论:miR-711 在胃癌细胞及组织中呈低表达,并与胃癌的分化程度、分期、淋巴结转移有关，提示miR-711 的表达异常与胃癌的发生发展相关。关键词:胃癌；miR-711；临床病理2ExpressionandclinicopathologicalcorrelationofmiR-711indifferenthumangastriccancercellsandtissues（Abstract）Objective:Toinvestigatetheexpressionandeffect of miR-711 in different human gastric cancer cells and tissues, then we explorethe clinical value of application preliminarily.Methods:Weculturedfourhuman gastric cancercellsofdifferentdegreeofdifferentiation:SGC-7901,BGC-823,AGS,MGC-803, and immortalized cells of normal gastricmucosa:GES-1. Wecollected28casesof gastriccancerpatients after surgeryto remove cancerous tissues and normal tissues. Detectedexpress- ion levels of miR-711 in gastric cancer cells and tissues byqRT-PCR, then we comparedthe expressionlevels ofdifference and analyzed ite correlationof clinicopathology.Results: Detected expressionlevels ofmiR-711byqRT-PCR, it showedthatallhumangastric cancer cells’ expression levels of miR-711 were less than expression levels intheGES-1 cells（P0.05）.MiR-711 expression in various cells showed thatGES-1 cells weremorethan SGC-7901 cells,and SGC-7901 cells were morethan BGC-823 cells, AGS cells,MGC-803 cells（P0.05）. MiR-711 expression level of BGC-823, AGS, MGC-803 gastric cancer cells w- erenosignificantdifference（P0.05）.Relativetonormal tissue,miR-711 expre- ssion level wassignificantlylower in gastriccancer tissues (P0.05).Its expre-ssion level wassignificantlyre