n-hacasio3复合根管封闭剂的体外评价word论文.docxVIP

摘要目的：观察纳米羟基磷灰石/硅酸钙复合生物材料的根尖封闭性能。方法：选取42 颗离体前牙，随机分为3 个试验组(每组各12 个)和2 个对照组(每组各3个)，采用逐步后退法预备根管，用侧向加压法进行充填。3个试验组所用根管材料分别是纳米羟基磷灰石/硅酸钙牙胶尖组(n-HA\Ca)、羟基磷灰石/硅酸钙复合材料糊剂加牙胶尖组(HA\Ca)、纳米羟基磷灰石加牙胶尖组(HA)，放置于恒温恒湿箱中一周，再用葡萄糖微渗漏测量法和扫描电镜观察根充材料与根管壁的密合度，评价根尖封闭性。结果：阴性对照组在一个月内未曾检测出葡萄糖的渗漏，而阳性对照组则于从第一天起就检测到的葡萄糖，并且呈快速上升的趋势.3个实验组两两比较，在1,4，7天检测出的渗漏吸光光度值差异无统计学意义（P﹥0.05），从第10天开始各组渗漏吸光光度值差距逐渐增大，且HA\Ca组的渗漏吸光光度值高于n-HA\Ca组，然而HA\Ca组和n-HA组之间差异无统计学意义，HA\Ca与n-HA\Ca组差异有统计学意义（P﹤0.05），从15天开始三组之间差异均有统计学意义。在20天开始HA\Ca组和n-HA组差异又开始无统计学意义，总的看来，通过苯酚硫酸法检测3组实验组和两个对照组的材料的葡萄糖渗漏量有显著性差异(T值：2.4647，P0.05) 结论：纳米羟基磷灰石/硅酸钙复合材料组的根尖封闭性能优于羟基磷灰石/硅酸钙复合材料组和纳米羟基磷灰石组。【关键词】;硅酸钙;纳米羟基磷灰石;微渗漏;密合度;葡萄糖测量法-1-AbstractObjectiveToobservetheapicalsealingabilityofnHA/CaSiO3compositematerials.MethodsForty-twostraightrootcanalteethwereusedinthisstudy.Theteethwererandomly dividedintothreetestgroups,12ineachgroup:(1)Nanohydroxyapatite/calciumsilicatetooth rubbertipgroup(n-HA\Ca);(2)Hydroxyapatite/calciumsilicatecompositetoothpasteplus toothrubbertipgroup(HA\Ca);(3)Nanohydroxyapatitewithrubbertipgroup(HA).In addition,therewerepositiveandnegativecontrolgroups(3teetheach).Stepwiseregressive methodwas used to preparethe rootcanal.Lateralcompression methodwasusedfor rootcanal filling.Glucosemicroleakagemeasurementandscanningelectronmicroscopy(SEM)were performedtoobservethesealtightnessofrootfillingmaterialandrootcanalwall,andto evaluatetheapicalsealingability.ResultsNegativecontrolgroupwasnotdetectedtheleakageofglucosewithin30days,In positivecontrolgroupwasdetectedhighconcentrationsofglucose,andrapidlyrising trend.from first day. Threeexperimental pairwisecomparison, in 1,4,7 days, thevalueofmicro leakagewasnostatistically significant(P0.05). starting from the 10th day,thevalueofeach team gap waswidening,and theHA\Cagroup is higherthanthen-HA\Cagroup, however there was nostatisticallysignificant differencebetween then-HAgroupandthe HA\Cagroup,HA/Caandn-HA/Cagroupwerestatisticallysignificantdifference(P﹤0.05),thestartingfrom15days,Thedifferencebetweenthre