rada16-nbd自组装多肽纳米支架对炎症刺激下成骨细胞影响的研究word论文.docxVIP

摘要目的：本研究是依据骨基质蛋白结构和功能的研究，通过改变氨基酸序列的方式， 在 RADA16 的基础上合成具有针对成骨细胞双重作用的多肽（RADA16-NBD）,并检测其对 炎症刺激下成骨细胞迁移、增值能力的影响。为氨基酸多肽的生物学应用以及临床研究 奠定了基础。方法：复苏成骨细胞株以及传代培养；通过混合法制备出 RADA16-NBD 水凝胶并用 扫描电镜观察其结构特点；同时在多肽水凝胶支架 RADA16-NBD 上接种炎症刺激下的成 骨细胞；采用划痕法观察水凝胶对炎症刺激下的成骨细胞迁移能力的影响；通过用CCK-8 检测多肽支架对炎症刺激下的成骨细胞的粘附和增值情况。 结果：通过扫描电镜可以看出细胞在水凝胶中得到了良好的伸展；CCK-8 的结果显示炎症刺激下的成骨细胞能够在 RADA16-NBD 水凝胶中良好的粘附、和增值；划痕实验 结果可看出 RADA16-NBD 水凝胶对炎症刺激下成骨细胞的迁移能力有较高的表达。结论：自组装多肽水凝胶（RADA16-NBD）能够支持炎症刺激下成骨细胞的粘附、伸 展、迁移和增殖，并体现出较高的表达。显示其既具有成骨又能抑制炎症活化的双重功 能，为应用于炎症性骨吸收特别是牙周炎引起的骨缺损的治疗开拓一条新的方向和思 路。同时为该实验的后续动物实验奠定了基础。关键词：多肽纳米支架；RADA16-NBD；成骨细胞；组织工程- 1 -AbstractObjective：According to the research of bone matrix’s structure and function. the research aims to synthesize polypeptide(RADA16-NBD)which has dual functions onosteoblasts, and to detect its effect on the migration、proliferation of inflammatory osteoblasst,thus lays foundation for the application of amino polypeptide in biology and clinical research. Polypeptide(RADA16-NBD)is synthesized By the method of changing the amino acid sequence and on the foundation of RADA16.Methods：Resuscitate osteoblast cell lines and subculture; Prepare hydrogel RADA16-NBD by mixing method and observe its structure by scanning electron microscopy. At the same time inoculate inflammatory osteoblasts on peptide hydrogel bracket RADA16-NBD; Observe the migration ability of osteoblast by the method of scarification; Detect the effect of peptide bracket on the adhesion and proliferation ability of inflammatory osteoblast cells by usingCCK-8.Result：Through scanning electron microscopy , it is seen that cells get good extension in hydrogel. CCK-8 shows that inflammatory osteoblasts have good adhesion and proliferation ability in hydrogel RADA16-NBD. The scarification experiment shows that hydrogel RADA16-NBD have a strong effect ion on the migration ability of inflammatory osteoblasts.Conclusion：Self-assembling peptide hydrogel can support the adhesion、spreading、migration and proliferation of inflammatory