人CRSCR3蛋白对急性大鼠脑缺血再灌注损伤的保护性试验研究.DOCVIP

人CR1-SCR1-3蛋白对急性大鼠脑缺血再灌注损伤的保护性实验研究 摘要：目的 探讨补体活化在大鼠急性脑缺血再灌注中的作用并观察人补体受体1型SCR1-3蛋白的保护作用。方法 将75只健康雄性SD大鼠，随机分为假手术组、CI/R组和CI/RCR1-SCR1-3组。采用线栓法建立大鼠大脑中动脉栓塞模型，缺血1h，再灌注24h，对大鼠神经功能缺陷进行评分，测定脑梗死体积，大脑皮层髓过氧化物酶 (MPO)活性、丙二醇(MDA)含量及超氧化物歧化酶(SOD)活性，观察大脑皮质区补体C4b沉积及病理改变。结果 缺血/再灌注24 h后，CR1-SCR1-3蛋白可改善保护组神经功能，明显减少保护组脑梗死体积，降低MPO、MDA水平并增高SOD活性；CI/RCR1-SCR1-3组缺血脑组织皮质区原位补体C4b沉积减少，病理损伤明显减轻。结论 补体活化参与了脑缺血再灌注损伤过程，CR1-SCR1-3蛋白对大鼠急性CI/R损伤具有保护作用。 关键词：补体；人补体受体1型；短同源重复1-3；大脑中动脉栓塞；脑缺血再灌注损伤；炎症 rotective effect of the SCR1-3 domain of complement receptor type 1 on acute cerebral ischemia and reperfusion injury Yang Shaojun, Zhang Xuan, Lu Yanzhi, Wang Zhengqing (Department of Microbiology,College of Basic Medical Sciences, Third Military Medical University,Chongqing 400038,China) ABSTRACT Objective: To investigate the effect of complement on acute cerebral ischemia and reperfusion injury and protection by CR1-SCR1-3 protein. Methods: A total of 75 adult male Sprague-Dawley rats were randomly divided into three groups: sham operation (SO) group (n=15); CI/R injury group (n=30); and CI/R group treated with CR1-SCR1-3 protein (n=30). Transient focal cerebral ischemia was induced by intraluminal MCAO method. After MCAO for 1h and reperfusion for 24h, Effects of CR1-SCR1-3 protein on neurological motor deficits, cerebral infarct size, three biochemical parameters including Myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD) in cerebral cortex were determined in this rat model. Meanwhile, the deposition of C4b in cerebral cortex and changes in pathological damage to cerebral tissue were also assessed. Results: 24h after MCAO and reperfusion, CR1-SCR1-3 could improve neurological functions and lead to a reduction in cerebral infarct size. Besides, pretreatment using CR1-SCR1-3 could decrease the MPO activity and the content of MDA, and increase the SOD activity. Decreased C4b deposition as well as improved morphological changes, were also observed in cerebral tissues of CI/R+CR1-SCR1-3