[临床医学]心血管病研究进展.ppt

* 1998年，我们教研室的肖献忠教授在美国首次制备出热休克因子1基因敲除小鼠，这就是热休克因子1基因敲除小鼠。 * * 1.更多的甚至更加关键的HSPs 作用的靶分子有待发现 2. 转基因技术在心肌保护临床应用中的理论和技术问题有待解决。 * * * * * * * * * * Identification of IPC-mediated differential protein by 2-DE and MS in rats. Table 3. identification of differential protein spots by MS 核仁素（Nucleolin）与心肌保护 Nucleolin is a multifunctional shuttle protein. Major functions: Binding and transporting rRNAs; Regulating the assembly of ribosomes; Regulating the growth and proliferation of cells; Virus infection; Tumor formation. 1. Nucleolin played important roles in oxidative stress induced cellular injuries; 2. Heat shock and heat shock protein 70 could inhibit the downregulation of nucleolin expression. Our studies: These results suggest that nucleolin may be an important injury-protecting factor. However, whether nucleolin has effects on IP and what the effects are, remain unclear. Fig1.5.The protective effect of myocardial ischemia preconditioning (IP) against the cleavage and down-regulation of nucleolin induced by ischemia/reperfusion injury. Fig1.4. The expression of nucleolin in rat myocardial ischemia preconditioning (IP). A:Western-blot experiments. B: Ratio of density of nucleolin bands to GAPDH bands (* P0.01 vs Sham group, n=3). Fig2.9. Effect of 100uM H2O2 preconditioning on the location of nucleolin protein. Fig2.10.Immunofluorescence analysis demonstrating the effect of H2O2 preconditioning on the location of nucleolin in cardiomyocytes. Ctrl H2O2-PC 12h Fig3.10. Percentage of apoptotic cells analysis demonstrating the effect of nucleolin ablation on protective effect of H2O2 preconditioning. (* P0.01 vs PsiRNA + H2O2 group;# P0.01 vs PsiRNA + H2O2-PC+ H2O2 group, n=6). Fig.4.8. The role of amino acid 310-713 of nucleolin in Hsp32 protein expression induced by nucleolin. Nuc1-309: Cells transfected with pEGFP-N1-Nuc1-309 p