紫杉醇及紫杉烷的细胞工程生产.pdfVIP

中国农学通报 2010,26(13):53-57 Chinese Agricultural Science Bulletin ·53 · 紫杉醇及紫杉烷的细胞工程生产 1,2 2 1,2 1 高明波 ，李兴泰 ，阮成江 ，范圣第 1 ( 生物化学工程国家民委－教育部重点实验室，辽宁大连116023； 2 大连民族学院生命科学学院，辽宁大连116600） 摘 要：紫杉醇是重要的抗癌药物，紫杉烷是紫杉醇的代谢前体或支路代谢产物，具有特殊生理活性，但 药源问题是制约紫杉醇及紫杉烷应用的主要因素。归纳了紫杉醇及紫杉烷的各种生产技术，指出了直 接提取及化学合成等方法的问题和细胞工程技术的优势。细胞工程技术环境友好，可调控，符合可持 续发展战略，生产紫杉醇及紫杉烷富有潜力。综述了紫杉醇及紫杉烷的细胞工程生产情况及各种策 略，指出了调控中忽视后生物合成过程的问题，提出了联合调控提高产量等策略及建议。 关键词：紫杉醇；紫杉烷；细胞工程；研究进展 中图分类号：Q813.1+ 1 文献标志码：A 论文编号：2009-2706 Production of Taxol and Taxanes by Cell Engineering 1,2 2 1,2 1 Gao Mingbo , Li Xingtai , Ruan Chengjiang , Fan Shengdi 1 ( Key Laboratory of Biochemical Engineering (SEAC-ME), Dalian Liaoning 116023; 2 College of Life Science, Dalian Nationalities University, Dalian Liaoning 116600) Abstract: Taxol is an important anticancer drug and taxanes are metabolic precursors or branch metabolites of taxol with special physiological activity. Pharmaceutical source is the main problem that restricts their application. Techniques to produce taxol and taxanes were summarized. Both disadvantages of direct extraction with chemical synthesis and advantages of cell engineering were indicated. Cell engineering is a potential technique to produce taxol and taxanes, being environmentally friendly, easy to control, in accordance with the strategy of sustainable development. Production of taxol and t