间接酶联免疫法检测鲑鳟鱼类传染性造血器官坏死病病毒.pdf

5 8 Vol. 5 No. 8 2014 8 Journal of Food Safety and Quality Aug. , 2014 1 1 2 2 2 1 金 爽 , 金莉莉 , 郭欣硕 , 蔺翠翠 , 罗 靳 , 王秋雨 (1. , 110036; 2. , 110031) 摘 要: 目的 (IHNV)方法 G (IHNV-G), , IHNV , ELISA , PCR 结果 ELISA , IHNV-G IHNV 1:32000 1:16000, PCR 98% 结论 , IHNV 关键词: ; ; ; Identification of infectious hematopoietic necrosis virus in trout by indirect-enzyme-linked immune assays 1 1 2 2 JIN Shuang , JIN Li-Li , GUO Xin-Shuo , LIN Cui-Cui , LUO Jin2 , WANG Qiu-Yu1 (1. College of Life Science, Liaoning University, Shenyang 110036, China; 2. Bureau of Testing on Aquatic Products Quality Safety of Liaoning Province, Shenyang 110031, China) ABSTRACT: Objective To establish an indirect enzyme-linked immune assays (ELISA) for the identification of infectious hematopoietic necrosis virus (IHNV). Methods An experimental rabbit was immunized to produce anti-IHNV serum with IHNV-G protein. To assess the titer and specificity of the anti-serum, the ELISA test was used. Further, PCR was used to verify the above ELISA experimental result. Results The titer of anti-serum of IHNV-G and IHNV reached to 1:32000 and 1:16000, respec