番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响27.pdfVIP

中国公共卫生2007年1月第23卷第1期 ChinJPublic月ealth]an2007Vol.23No.1 文章编号:1001-0580(2007)01-0093-03 中图分类号:R96 文献标志码:A 实【验研究】 番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响 ‘ 董殿军，林晓明 摘 要:目的 观察番茄红素对急性肺损伤大鼠肺泡巨噬细胞吞噬功能的影响。方法 以0.5,1.0,3.0mg/(kg .bw)的番茄红素连续给雄性Wistar大鼠灌胃30d，用脂多糖制造大鼠急性肺损伤模型，分别在1,4,6h后支气管肺泡 灌洗，检测肺泡巨噬细胞吞噬功能、细胞计数以及肺相对重等指标。结果 在各剂量番茄红素作用下，肺泡巨噬细胞 吞噬功能明显增强，与模型组相比，注射脂多糖1h后，喂饲番茄红素组吞噬功能增强，增强率分别为11.2%,27.9%, 38.1%;4h后，增强率分别为5.9%,4.4%,17.0%;6h后，增强率分别为16.6%,15.2%和21.9%。番茄红素3个剂 量组之间呈剂量一反应关系(P0.05)。结论 番茄红素在0.5,1.0,3.0mg/(掩.bw)剂量下能增强肺泡巨噬细胞的 吞噬功能。 关锐词:番茄红素;急性肺损伤;肺泡巨噬细胞;吞噬功能 Effectoflycopeneonfunctionofpulmonaryalveolarmacrophagesofratsacutelungi川uryDONGDan一un,LINXia-o ming.DepartmentofNutritionandFoodHygiene,SchoolofPublicHealth,PekingUniversity(Beijing100083,China) Abstract:ObjectiveTostudytheeffectoflycopeneonthefunctionofpulmonaryalveolarmacrophages(PAMs)ofrat sacutelunginjury(ALI).Methods Maleratsweregavagedcontinuouslybylycopenewith0.5,1.0,3.0mg/kgfor30 days;LipopolysaccharidewasusedtomakethemodelofALIrats.After1hour,4and6hours,broncboalveolarlavagewas done,andthefunctionofPAMs,theamountofPAMsandthelungrelativeweightweredetermined.Results Lycopenecould increasethephagocyticfunctionofPAMssignificantly.Comparedwiththemodelgroup,thephagocyticfunctionofPAMs wasincreasedsignificantlyinlycopenegroups,withincreasingratesof11.2%，27.9%，38.1%afterinjectionofLPSfor1 hour,withincreasingratesof5.9%，4.4%，17.0%afterinjectionofLPSfor4hours,andwithincreasingratesof16.6%， 15.2%，21.9%afterinjectionofLPSfor6hours,respectivelelycomparedwiththemodelgroup.Thephagocyticfunctionof PAMswasincreasedobviouslybythreedifferentdoseoflycopene,showingadose-responserelationship(P0.05).Conclu- sion Lycopene,atthedoseof0.5,1.0and3.0mg/kg,couldreinforcethephagocyticfunctionofPAMs. Keywords:lycopene;acutelunginjury(ALI);pulmonaryalveolarmacrophages(PAMs);p