利用套叠PCR技术改造酵母表达载体pAO815的研究-生命科学研究.PDFVIP

6 4 生命科学研究 Vol.6 No.4 2002 12 Life Science Research Dec. 2002 PCR pAO815 周 鹏, 郭安平, 黎小瑛 ( , 571101) : 利用套叠 PCR 技术将factor 信 号肽基因插入 pAO815 之EcoR 位点, 构建成分泌型 达载体 pAO815A. 在不改变原克隆位点 EcoR 的条件下, 借助pAO815 之第873 位的Hind 位点, 将 pAO815 AOX1 启动子873940 片段连同factor 信号肽基因序列 ( 246 bp) 插入pAO815 之Hind EcoR 之间, 构建成含 factor 信号肽基因的分泌型 达载体pAO815A. : 套叠PCR; 酵母 达载体pAO815;分泌型 达载体 : Q781 :A :2002) Reconstruction of pAO815 of Yeast Expression Vector by Overlap Extension HOU Peng,GUO Anping,LI Xiaoying ( State Key Laboratoryf or Trop ical Crops Biotechnology , CA TAS, Haikou 571101,H ainan , China) Abstract: The pAO815A of secrete expression vector was constructed with factor signal peptide gene inserted into EcoR site of pAO815 by overlap extension of PCR technique,which 873th940th bps fragment of pAO815 AOX1promoter with factor signal peptide gene was inserted into H ind EcoR site and a kind of pAO815 containing factor signal peptide gene was constructed under nonchanging previous EcoR cloning site. Key words: overlap extension of PCR; pAO815 of yeast expression vector; secretetype expression vector (Lfi e Science Research, 2002, 6(4) : 310~ 313) ( Pichia p astoris ) . , PCR factor pAO815 YIP , EcoR , , , YIP EcoR , pAO815 [ 1~ 3] , . 873 H ind , pAO815AOX1 pAO815