毕业设计（论文）-玉米多糖提取纯化及其性质研究精选.doc

学位论文原创性声明 本人郑重声明：所呈交的论文是本人在导师的指导下独立进行研究所取得的研究成果。除了文中特别加以标注引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写的成果作品。本人完全意识到本声明的法律后果由本人承担。 作者签名： 年 月 日 学位论文版权使用授权书 本学位论文作者完全了解学院有关保障、使用学位论文的规定，同意学院保留并向有关学位论文管理部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权省级优秀学士学位论文评选机构将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。 本学位论文属于 1、必威体育官网网址 □，在_________年解密后适用本授权书。 2、不必威体育官网网址 □。 （请在以上相应方框内打“√”） 作者签名： 年 月 日 导师签名： 年 月 日  要多糖(Polysaccharides)是含酮基或醛基的大分子多聚物，并研究纯化后的玉米多糖的抗氧化性、絮凝性、抑菌性。单因素实验结果：最佳提取工艺是按料液比1:20，在90o?的条件下提取2h，重复提取2次。其最大提取率为14.86%；研究发现玉米多糖对羟基自由基（OH）的清除率可达42.6%，在对DPPH的清除率可达65.1%，其总体抗氧化性能低于；絮凝性实验发现，玉米多糖有较好的絮凝效果，絮凝率可达49.2%；最后抑菌性实验表明，玉米多糖对枯草芽孢杆菌、金黄色葡萄球菌有较好的抑菌表现，对大肠杆菌的抑菌性较低，对黑曲霉没有抑菌性，MIC为6.25mg/mL。 关键词：玉米多糖；提取；纯化；抗氧化；絮凝性；抑菌性 Abstract Polysaccharides were natural polymer micelle which contains ketone group or aldehyde group, it basic unit was monosaccharide. In this article, the material is corn, polysaccharide extracted by the water extraction and ethyl alcohol precipitation method. The content of polysaccharide was detecteded by phenol-sulfuric acid method, and the content of protein was detecteded by bandford method. The crude corn polysaccharide were removed the protein by Sevage method and researched the antioxidant activity, flocculating peopety, antimicrobial activity of corn polysaccharide. Through the single factor experiment, found the best condition was the material fluid ratio is 1:20, extracts temperature was 90o?, extracts time was 2h, extracts 2 times in total, the corn polysaccharide extraction rate as much as 14.86%. In this study, the result showed that the polysaccharide on hydroxyl free radical (?OH) removal effect reached 42.6%, the highest DPPH? free radical (DPPH?) scavenging effect reached 65.1%. Through comparing with Vc, the polysaccharide on free radical clearance rate was lower. In the study of flocculating peopety, found that corn polysaccharide had good flocculating peopety, for caolin it could reach 49.2% and for CaO it could reach 57.4%.