HPLC同时测定大豆磷脂中磷脂酰胆碱和溶血磷脂.pdf

HPLC 同时测定大豆磷脂中磷脂酰胆碱和溶血磷脂酰胆碱含量 王剑霞1 1 1 1 2* ，黄光亮 ，陈禧翎 ，段敏丽 ，罗昕 (1. 广州白云山明兴制药有限公司，广州 510250 ；2. 中山大学药学院，广州 510006) 摘要：目的 建立同时测定大豆磷脂中磷脂酰胆碱(PC)和溶血磷脂酰胆碱(LPC)含量的方法。方法 采用高效液相色谱法， 色谱柱为Phenomenex silica 柱(4.6 mm×150 mm ，5 μm) ，流动相为正己烷-异丙醇-水(5 ∶80 ∶20) ，流速：0.7 mL·min1 ， 检测波长：206 nm ，柱温：25 ℃。结果 PC 在 0.4~2.4 mg·mL1 内呈良好的线性关系，平均回收率为99.4% ，RSD 为 0.40%(n=6) ；LPC 在 10~60 μg·mL1 内呈良好的线性关系，平均回收率为98.0% ，RSD 为1.65%(n=6) 。结论 本试验方法 简便、重复性好、专属性强，可作为大豆磷脂中PC ，LPC 的检测方法。 关键词：大豆磷脂；磷脂酰胆碱；溶血磷脂酰胆碱；含量测定 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2011)06-0564-03 Determination of Phosphatidylcholine and Lysophosphatidylcholine in Soybean Phospholipids by HPLC 1 1 1 1 2* WANG Jianxia , HUANG Guangliang , CHEN Xiling , Duan Minli , LUO Xin (1.Guangzhou Baiyunshan Mingxing Pharmaceutical Co., Ltd., Guangzhou 510250, China; 2.School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China) ABSTRACT: OBJECTIVE To establish an HPLC method for simultaneously determining phosphatidylcholine (PC), lysophosphatidylcholine (LPC) in soybean phospholipids. METHODS The HPLC system consisted of a Phenomenex silica column(4.6 mm×150 mm, 5 μm), a mobile phase of n-hexane-isopropanol-water(5 ∶80 ∶20). The flow rate was 0.7 mL·min1. Column temperature was set at 25 ℃and the wavelength of detector was set at 206 nm. RESULTS The calibration curve was linear in the range of 0.42.4 mg·mL1 for PC and 1060 μg·mL1 for LPC, respectively. The average recovery for PC was 99.4% and RSD was 0.40%(n=6). The average recovery for LPC was 98.0% and RSD was 1.65%(n=6). CONCLUSION The method is simple, rapid and specially. It can be used for the determination of PC and LPC in soybean phospholipids. KEY WORDS: soybean phosphol