HPLC法测定5种1变种金银花中绿原酸含量.pdf

2004年 5月第 11卷第 5期 中国中医药信息杂志 ·421· HPLC 法测定5 种1 变种金银花中绿原酸含量 1 2 苟占平 ,万德光 (1.暨南大学药学院,广东 广州 510632；2.成都中医药大学药学院,四川 成都 611731) 摘要：目的 以绿原酸含量为指标,评价测定川渝产金银花的质量。方法 采用 HPLC 法。结果 四川旺苍和成 都产正品中绿原酸的含量分别为 0.64%和 0.58%,远低于 2000 年版《中国药典》(一部)规定的标准(≥1.5%),约为河 南密县道地金银花 3.02%的 1/5。其余川渝所产金银花样品(4 种 1 变种)的绿原酸含量大多在 1%以上,其中淡红忍冬 L. acuminata Wall.、峨眉忍冬 L.similis Hemsl.var.omeiensis Hsu et H.J.Wang 中绿原酸含量为首次用 HPLC 法测定报道。康定产的淡红忍冬 L.acuminata Wall.绿原酸含量 0.47%约为沐川 3.01%的 1/6。结论 同种忍冬不同 产地花蕾样品的绿原酸含量差异很大,等于甚至大于不同品种间的差异,四川正品金银花远不及河南。说明提倡和发 展金银花道地药材生产是完全必要的。 关键词：金银花；绿原酸；含量测定；高效液相色谱法 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2004)05-0421-03 Determination of Chlorogenic Acid in 5 Species and 1 Variety of Flos Lonicerae by HPLC GOU Zhan-ping1, WAN De-guang2 (1.Pharmaceutic College of Jinan University, Guangzhou 510632, China；2.Pharmaceutic College of Chengdu University of TCM, Chengdu 611731, China) Abstract ：Objective To determine the content of chlorogenic acid in Flos Lonicerae in Sichuan province and Chongqing city for evaluating the quality of them. Method The HPLC analysis was used. Result The content of chlorogenic acid in samples of Lonicera japonica Thunb. in Wangcang county and Chengdu city is 0.64% and 0.58% respectively, which is far lower than the criterion (≥1.5%) provided in 2000 years edition Chinese pharmacopoeia and only about 1/5 as high as it is 3.02% in sample of Lonicera japonica Thunb. in Mixian county. It is higher than 1% in samples of other 4 species and 1 variety in Sichuan province and Chongqing city for the most part and was determined by HPLC and reported for the first time for L. acuminata Wall. and L. similis Hemsl. var. omeiensis Hsu et H. J. Wang. In sample of L