基于matK序列的木犀属植物系统发育初步研究.docx

第 31 卷 第 6期2009 年11月北 京 林 业 大 学 学 报JOURNAL OF BEIJ ING FORESTRYUNIVERSITYVol. 31 , No.6Nov. ,2009基于 matK序列的木犀属植物系统发育初步研究杨绪勤邓传良刘丽盈沙涛于美玲卢龙斗(河南师范大学生命科学学院)摘要:以流苏树属的流苏树,女贞属的女贞和木犀榄属的木犀榄作为外类群,利用叶绿体matK基因序列,对木犀属19个种进行种间亲缘关系及系统分类学研究。结果表明:①木犀属植物叶绿体matK基因序列较为保守,特别表现在3′端;②木犀属植物是一单系类群;③19种木犀属植物可以分为3个分支:香花木犀和华东木犀各单独聚为一个分支;其余聚为一个分支;其中美洲木犀、牛矢果和厚边木犀聚为一个亚分支,都属于圆锥花序组;木犀组的红柄木犀、毛木犀、石山桂、狭叶木犀和管花木犀组的山桂花5种植物聚为一个亚分支;其余的9种木犀组植物聚为一 个亚分支;④经典的基于形态学的P. S.Green分类系统没有得到分子数据的支持,圆锥花序组聚在一起,而木犀组 和管花木犀组之间的亲缘关系是交叉的。因此作者认为matK序列需要结合其他分子序列以及形态学指标进行综合分析,才能获得科学合理的木犀属分类系统。关键词:木犀属; matK基因;系统发育分析中图分类号 : Q94411 ; S71813 文献标志码 :A 文章编号 :1000221522 (2009)062200092206YANG Xu2qin ; DENG Chuan2liang ; L IU Li2ying ; SHA Tao ; YUMei2ling ; LU Long2dou.Phylogenetic analysis of genus Osmanthus (Oleaceae) based on mat K sequence. Journal of Beijing Forestry University(2009) 31 ( 6 ) 92214 [ Ch , 33 ref . ] College of Life Science , Henan Normal University , Xinxiang City,453007 , P. R. China.Phylogenetic analysis of genus Osmanthus was performed based on sequence data of the chloroplast matK gene in 19 species and outgroups ( Chionanthus retusus , Ligustrim lucidum and Olea europaea ) . Theresultsshowed that : 1) sequence of the chloroplast mat K gene in Osmanthus was more conservative , especially in3′terminal ; 2) the genus Osmanthus was a monophyletic group ; 3) 19 species of Osmanthus were divided into three clades : O . suavis and O . cooperi constitute as a monophyletic clade ; the others constitute as a clade :O.americanus,O.matsumuranusandO.marginatusconstituteasasubclade,belongingtoSect.Leiole. Another subclade was constituted by O . armatus , O. venosus , O . fordii , O . attenuatus and O. delarayi;the other subclade was constituted by the remaining 9 species , belonging to Sect. Osmanthus ; 4) the studyresult showed that the classical classification system of P. S. Greenπs based on morphology couldn’t besupported by m