复合基质生物产氢机制及程序应用之整合研究.pdf

複合基質生物產氫機制及程序應用之整合研究-子計畫三 計畫編號 : NSC90-2211-E006-052 執行期限:90/8/1～91/7/31 主持人:曾怡禎國立成功大學 生物系 一、中文摘要 indigenous Clostridia and physiological characteristics of hydrogen production. 本研究之主要目的是希望能從不 71 strains were isolated from composts, 同來源樣本分離出高效能的產氫菌 rice shell, food and wine wastewater 株 ，研究高效能產氫菌株的生理特性 treatment plants, but only 27 strains can produce hydrogen. C008 strain was 以作為調控其產氫能力的基礎。本研 isolated from compost with optimum 究自稻草堆肥、牛糞堆肥、稻殼和食 growth at 30 and pH 7.0. The enhance 品及酒廠廢水處理廠共分離出 71 株菌 of hydrogen production was achieved by 株，其中27 株具有產氫能力，但其差 combination of glucose and yeast extract 異相當大。 分離自牛糞堆肥的菌株 as the growth medium. The hydrogen C008 為產氫活性最高的菌株，其氫轉 production potential of Clostridium 換率為1.39mole H /mole Glucose 。分 butyricum and C008 strain was 1.39 and 2 2.86 mole hydrogen/mole glucose, 離菌株C008 經由16S rDNA 序列比對 respectively. The fermentation products 的結果，其親源關係與 Clostridium of C008, acetate and butyrate were tyrobutyricum最為相近。C008菌株屬 produced in the ratio of greater than 1 於發酵產氫菌，在葡萄糖含 Yeast during the 20 hours growth. The extract或 Peptone的複合基質其產氫量 transition from the acidiogenic phase to 顯著的高於只含葡萄糖的單一基質。 solventogenic phase was not observed C008菌株在發酵產氫時的產物以乙酸 during the growth of C008 strain. The 和丁酸為主