偏硅酸钠对ECV304细胞株与单个核细胞黏附的影响.doc

偏硅酸钠对ECV304细胞株与单个核细胞黏附的影响作者：黄伟 李丽玮 甄艳军 李志永 张 莉 侯建明????作者单位：河北医科大学西校区基础课教学部,河北 石家庄 050091【摘要】? 目的 观察偏硅酸钠(Na2SiO3)对氧化低密度脂蛋白(oxLDL)诱导的ECV304细胞与人外周血单个核细胞黏附的影响，探讨其抗动脉粥样硬化（AS）作用的机制。方法 ECV304细胞分为5组:对照组、oxLDL组、Na2SiO3高剂量组（硅68.5 mg/L）、中剂量组(硅34.2 mg/L)、低剂量组(硅17.1 mg/L)。分离正常健康人外周血单个核细胞，计数与各组ECV304细胞黏附数，检测细胞培养液中IL8水平；采用流式细胞术测VCAM1的蛋白表达量；半定量RTPCR检测VCAM1、IL8mRNA的表达。结果 Na2SiO3减少ECV304单个核细胞黏附数(P＜0.01)，降低IL8浓度(P＜0.01)，降低VCAM1的蛋白表达量(P＜0.05)，下调VCAM1、IL8 mRNA的表达(P＜0.01)。结论 Na2SiO3能够下调oxLDL诱导的ECV304 IL8和VCAM1的表达。 【关键词】? 偏硅酸钠;氧化低密度脂蛋白；ECV304；VCAM1；白细胞介素8 Effect of Na2SiO3 on mononuclear cells adhesion to ECV304 cells induced by oxidized low density lipoprotein ??? HUANG Wei, LI LiWei, ZHEN YanJun, et al. ??? Hebei Medical University, Shijiazhuang 050091, Hebei, China ??? 【Abstract】? Objective? To observe the effect of Na2SiO3 on mononuclear cells adhesion to ECV304 cells induced by oxidized low density lipoprotein (oxLDL). Methods? Logarithmic growth phase of ECV304 cells were divided into control, oxLDL, high,middle and low dose（Si 68.5, 34.2, 17.1 mg/L）of? Na2SiO3 groups. The level of interleukin8(IL8)in cell culture fluid was tested, the protein expression of vascular cellular adhesion molecule (VCAM)1 was measured by flow cytometry, the gene expressions of VCAM1 and IL8 were measured by half quantitative reverse transcription polymerase chain reaction (RTPCR). Results? Na2SiO3 decreased the level of IL8 and the number of mononuclear cell adhesion(P＜0.01),and the expressions of VCAM1 protein（P＜0.05), VCAM1 gene(P＜0.01) and IL8 gene(P＜0.01).Conclusions? Na2SiO3 can inhibit the expression of adhesion molecule in ECV304 cells induced by oxLDL. ??? 【Key words】? Na2SiO3; Oxidized low density lipoprotein; ECV304; Vascular cellular adhesion molecule1; Interleukin8 ??? 文献报道可溶性硅化物，如硅酸、偏硅酸，有降血脂、防治动脉粥样硬化(AS)的作用。本课题组曾用整体动物做实验，结果表明偏硅酸钠（Na2SiO3）能从许多环节抑制高脂血症条件下AS的形成和发展,如Na2SiO3可下调白细胞黏附分子的表达、降低血清IL8含量、纠正NO代谢紊乱等〔1，2〕,提示Na2SiO3在抗AS作用方面有潜在重要研究价值。但是有关Na2SiO3